Gut Microbiota Composition Changes in Constipated Women of Reproductive Age

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Reviewed Marked as Reviewed by Svetlana up on 2024-5-13
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Li H, Chen J, Ren X, Yang C, Liu S, Bai X, Shan S, Dong X
Journal
Frontiers in cellular and infection microbiology
Year
2020
Keywords:
16S rRNA gene sequencing, chronic constipation, gut microbiota, influence factors, women of reproductive age
BACKGROUND: Chronic constipation is one of the most prevalent functional gastrointestinal disorders, yet its etiology is multifactorial, and the pathophysiological mechanism is still unclear. Previous studies have shown that the gut microbiota of constipated patients differs from healthy controls; however, many discrepancies exist in the findings, and no clear link has been confirmed between chronic constipation and changes in the gut microbiota. Growing evidence indicates that age, gender, and hormone levels can affect the composition of gut microbiota. The aim of this study is to examine the overall changes in gut microbiota within a specific sub-population of patients, namely, constipated women of reproductive age. METHODS: We carried out a cross-sectional study comparing the fecal microbial composition of 30 healthy women and 29 constipated women using 16S rRNA gene sequencing. Only women of reproductive age were recruited to reduce the effects of age, gender, and hormone levels on the microbiome, and to prevent conflating the impact of these factors with the effects of constipation. RESULTS: There were obvious differences in the gut microbiota in constipated women of reproductive age compared with the healthy controls, manifesting mainly as a significant increase in the abundance of Bacteroides (p < 0.05) and a significant decrease in the abundance of Proteobacteria (p < 0.01). The overall composition of the gut microbiota in each group was different, which was reflected in the ratios of Firmicutes to Bacteroidetes (F/B), which was 1.52 in the constipated group vs. 2.21 in the healthy group. Additionally, there was a significant decrease in butyrate-producing bacteria, like Roseburia and Fusicatenibacter (p < 0.01). CONCLUSION: The overall composition of the gut microbiota changed in constipated women of reproductive age, characterized by a loss in Proteobacteria and an increase in Bacteroidetes. Furthermore, the abundance of some butyrate-producing bacteria also reduced. These changes may reflect the unique interactions between host and some bacteria, or some bacterial metabolic products, which may be important targets for future studies to explore the pathogenesis of constipation.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-5-13

Curated date: 2024/04/26

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Chronic constipation Chronic constipation,Infrequent bowel movements,chronic constipation
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy women
Group 1 name Corresponds to the case (exposed) group for case-control studies
Constipated women
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Female constipated patients of reproductive age.
Group 0 sample size Number of subjects in the control (unexposed) group
30
Group 1 sample size Number of subjects in the case (exposed) group
29
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
One month preceding sample collection.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-5-13

Curated date: 2024/04/26

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2 and Figure3f

Description: Significant Differences in the Microbiota of Constipated Women of Reproductive Age and Healthy Controls.

Abundance in Group 1: increased abundance in Constipated women

NCBI Quality ControlLinks
Bacteroidaceae
Bacteroidales
Bacteroides
Bacteroidia
Bacteroidota
Negativicutes
Phocaeicola coprocola
Selenomonadales
Fusicatenibacter
Lactobacillaceae
Tannerellaceae
Parabacteroides
Citrobacter

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-5-13

Curated date: 2024/04/26

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2 and Figure3f

Description: Significant Differences in the Microbiota of Constipated Women of Reproductive Age and Healthy Controls.

Abundance in Group 1: decreased abundance in Constipated women

NCBI Quality ControlLinks
Enterobacterales
Enterobacteriaceae
Escherichia coli
Gammaproteobacteria
Klebsiella
Klebsiella pneumoniae
Pseudomonadota
unclassified Enterobacteriaceae
Roseburia

Revision editor(s): Aleru Divine