Microbiota in anorexia nervosa: The triangle between bacterial species, metabolites and psychological tests/Experiment 1

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Reviewed Marked as Reviewed by Fatima on 2021/07/8

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Rimsha, WikiWorks, Victoria

Subjects

Location of subjects
Italy
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Anorexia nervosa anorexia nervosa,Anorexia nervosa
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
Aonerxia Nervosa patients
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The severity of eating disorder and psychopathology tests were assessed by means of the Symptom Checklist 90 (general psychopathology), Eating Disorder Inventory 2 (eating disorder), State Trait Anxiety Inventory (anxiety disorder) and Beck Depression Inventory (depressive symptoms). Psychologists and psychiatrists (all extensively trained in the use of the instruments) conducted clinical evaluations.
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
15
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Matched on Factors on which subjects have been matched on in a case-control study
age, sex


Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Table 3 +text

Description: Significant differential abundance in anorexia nervosa vs controls

Abundance in Group 1: increased abundance in Aonerxia Nervosa patients

NCBI Quality ControlLinks
Pseudomonadota
Enterobacteriaceae
Methanobrevibacter smithii

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Table 3 +text

Description: Significant differential abundance in anorexia nervosa vs controls

Abundance in Group 1: decreased abundance in Aonerxia Nervosa patients

NCBI Quality ControlLinks
Bacillota
Oscillospiraceae
Ruminococcus
Roseburia
Clostridium

Revision editor(s): WikiWorks