Fecal microbiome signatures are different in food-allergic children compared to siblings and healthy children/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Food allergy Food Hypersensitivity,Food intolerance,Food allergy,food allergy
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- healthy sibling
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- sibling with no food allergy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 21
- Group 1 sample size Number of subjects in the case (exposed) group
- 25
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between non-food-allergic siblings and healthy controls
Abundance in Group 1: increased abundance in healthy sibling
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillibacter valericigenes | ||
| Faecalibacterium sp. | ||
| Alistipes putredinis | ||
| Alistipes sp. |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-4
Source: Figure 3, Figure 4, Figure 5
Description: Differentially abundant operational taxonomic units (OTUs) obsereved between non-food-allergic siblings and healthy controls
Abundance in Group 1: decreased abundance in healthy sibling
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. | ||
| Haemophilus parainfluenzae |
