Changes in the stool and oropharyngeal microbiome in obsessive-compulsive disorder/Experiment 2

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-22

Curated date: 2023/03/24

Curator: Kahvecirem

Revision editor(s): Kahvecirem, Claregrieve1

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Oropharynx Mesopharynx,Oral part of pharynx,Pars oralis pharyngis,Oropharynx,oropharynx

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Obsessive-compulsive disorder Anancastic neurosis,obsessive compulsive disorder,obsessive-compulsive disorder,OCD,Obsessive-compulsive disorder

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Control (healthy group)

Group 1 name Corresponds to the case (exposed) group for case-control studies: OCD T0

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with Obsessive-compulsive disorder (OCD), which is a neuropsychiatric condition characterized by intrusive and unwanted thoughts (termed obsessions) and repetitive behaviors or mental acts (termed compulsions), which are carried out to at least partially alleviate the anxiety or distress brought on by the obsessions. Timepoint 0 was the baseline timepoint prior to three months of pharmacological treatment and cognitive behavioral therapy (referred to as T0).

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2.0

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa: unchanged

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample: unchanged

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-22

Curated date: 2023/03/24

Curator: Kahvecirem

Revision editor(s): Kahvecirem, Aiyshaaaa, Merit, Claregrieve1

Source: Figure 4A

Description: Differential microbial abundance in oropharyngeal samples between controls and T0 OCD patients

Abundance in Group 1: increased abundance in OCD T0