Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome/Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-24

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Claregrieve1

Subjects

Location of subjects
Canada
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Air pollution air pollution,Air pollution
Group 0 name Corresponds to the control (unexposed) group for case-control studies
healthy mice
Group 1 name Corresponds to the case (exposed) group for case-control studies
Chronic treatment group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
6-week old female wild-type 129/SvEv mice in chronic treatment group were fed mouse chow ± PM10 (0.09 gm/kg) for 35 days
Group 0 sample size Number of subjects in the control (unexposed) group
7
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Non-quantitative PCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
PLS-DA (Partial least square discriminant analysis)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-24

Curated date: 2021/01/10

Curator: Zyaijah Bailey

Revision editor(s): WikiWorks, Claregrieve1

Source: Figure 6

Description: Microbiota composition in stool samples from WT and IL-10−/− mice after 35 days of treatment with PM10.

Abundance in Group 1: increased abundance in Chronic treatment group

NCBI Quality ControlLinks
Verrucomicrobiota

Revision editor(s): WikiWorks, Claregrieve1