Microbiota and Derived Parameters in Fecal Samples of Infants with Non-IgE Cow's Milk Protein Allergy under a Restricted Diet/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Atrayees on 2023-8-3
Subjects
- Location of subjects
- Spain
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Milk allergic reaction milk allergic reaction,milk allergy,Milk Hypersensitivity,Milk allergic reaction
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- infant with non-IgE-mediated cow's milk allergy
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- infant with cow's milk protein allergy confirmed by a negative skin prick test, values lower than 0.35 kU/L cow’s milk-specific IgE determined in their blood, and a clear positive standardized oral challenge (SOC), performed under medical supervision
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 17
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 4 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Metastats
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- age
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-8-3
Source: Figure 2, text
Description: Differences in relative abundances (%) of sequences in fecal samples of infants with non-IgE mediated cow’s milk protein allergy (NIM-CMPA) and non-allergic control infants
Abundance in Group 1: increased abundance in infant with non-IgE-mediated cow's milk allergy
| NCBI | Quality Control | Links |
|---|---|---|
| Coriobacteriaceae |
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-8-3
Source: Figure 2
Description: Differences in relative abundances (%) of sequences in fecal samples of infants with non-IgE mediated cow’s milk protein allergy (NIM-CMPA) and non-allergic control infants
Abundance in Group 1: decreased abundance in infant with non-IgE-mediated cow's milk allergy
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidota | ||
| Bacteroidaceae | ||
| Bacteroides |
Revision editor(s): WikiWorks
