The Association Between Gestational Diabetes and Microbiota in Placenta and Cord Blood/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Peace Sandy on 2024-1-12
Curated date: 2021/06/14
Curator: Madhubani Dey
Revision editor(s): Madhubani Dey, WikiWorks, Peace Sandy
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Placenta Allantoic placenta,Eutherian placenta,Placenta,placenta
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- euglycemic (control) singleton pregnant women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Pregnant women with Gestational Diabetes Mellitus
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Pregnant women with Gestational Diabetes Mellitus (GDM) who had prenatal care at Xinhua Hospital, a tertiary hospital in Shanghai, and were admitted to the hospital for cesarean deliveries
- Group 0 sample size Number of subjects in the control (unexposed) group
- 7
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Women who were treated with any antibiotics during pregnancy
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-1-12
Source: Table 3
Description: Table 3: Relative abundances (%) of placental microbiota OTUs that differed between eight women with GDM and seven women without GDM (non-GDM).
Abundance in Group 1: increased abundance in Pregnant women with Gestational Diabetes Mellitus
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerotruncus | ||
| Coprococcus | ||
| Defluviitaleaceae | ||
| Dorea | ||
| Paraprevotella | ||
| Prevotella | ||
| Ruminococcus | ||
| unclassified Lachnospiraceae | ||
| uncultured Lachnospiraceae bacterium |
Revision editor(s): Madhubani Dey, Peace Sandy
Signature 2
Reviewed Marked as Reviewed by Peace Sandy on 2024-1-12
Source: Table 3
Description: Table 3: Relative abundances (%) of placental microbiota OTUs that differed between eight women with GDM and seven women without GDM (non-GDM).
Abundance in Group 1: decreased abundance in Pregnant women with Gestational Diabetes Mellitus
| NCBI | Quality Control | Links |
|---|---|---|
| Veillonella |
Revision editor(s): Madhubani Dey, Peace Sandy
