The role of the vaginal microbiome in distinguishing female chronic pelvic pain caused by endometriosis/adenomyosis/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Atrayees on 2023-7-17
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Posterior fornix of vagina Pars posterior fornicis vaginae,Posterior fornix,Posterior part of fornix of vagina,Posterior fornix of vagina,posterior fornix of vagina
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Women without CPPS presenting for routine examinations
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Women with EM-associated CPPS
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Thirty-seven women with endometriosis-associated chronic pelvic pain syndrome (CPPS) confirmed by exploratory laparoscopy or surgical pathology
- Group 0 sample size Number of subjects in the control (unexposed) group
- 66
- Group 1 sample size Number of subjects in the case (exposed) group
- 37
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Patients were excluded if they had taken antibiotics within 14 days prior to sampling
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
- Matched on Factors on which subjects have been matched on in a case-control study
- age
Alpha Diversity
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-7-17
Source: Table 2
Description: The following taxa were increased in women with endometriosis associated CPPS pain
Abundance in Group 1: increased abundance in Women with EM-associated CPPS
| NCBI | Quality Control | Links |
|---|---|---|
| Alloscardovia | ||
| Stenotrophomonas | ||
| Veillonella |
Revision editor(s): Samara.Khan, Atrayees
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-7-17
Source: Table 2
Description: The following taxa were decreased in women with endometriosis associated CPPS pain
Abundance in Group 1: decreased abundance in Women with EM-associated CPPS
| NCBI | Quality Control | Links |
|---|---|---|
| Megasphaera | ||
| Shuttleworthella | ||
| Sneathia |
Revision editor(s): Samara.Khan, Atrayees
