Improved feeding tolerance and growth are linked to increased gut microbial community diversity in very-low-birth-weight infants fed mother's own milk compared with donor breast milk/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Peace Sandy on 2024-2-21

Curated date: 2022/06/29

Curator: Uyokeeswaran

Revision editor(s): Uyokeeswaran, Aiyshaaaa, Atrayees

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Maternal milk mother's own milk,breast milk,Maternal milk,maternal milk
Group 0 name Corresponds to the control (unexposed) group for case-control studies
DM (Donor's Milk)
Group 1 name Corresponds to the case (exposed) group for case-control studies
MOM (Mother's own milk)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mother's own milk group consists of infants who drank at least >50% of mother's own milk. Any less and they were placed in the other cohort.
Group 0 sample size Number of subjects in the control (unexposed) group
43
Group 1 sample size Number of subjects in the case (exposed) group
74
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
No antibiotic exclusions were listed as infants were at a vulnerable stage in their lives.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Logistic Regression
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-21

Curated date: 2022/06/29

Curator: Uyokeeswaran

Revision editor(s): Uyokeeswaran, Peace Sandy

Source: FIGURE 2C and FIGURE 2 D

Description: (C) When comparing longitudinal changes across samples from all study subjects, increasing relative abundance of Proteobacteria was observed. There were no significant differences observed at the phylum level during the first 2 wk of life. By week 4, microbiota from the MOM cohort had significantly higher abundance of Actinobacteria (P = 0.032) and decreased abundance of Firmicutes (P = 0.011). (D) By week 4, microbiota from the MOM cohort had significantly increased abundance of Bacteroides (P = 0.046), Bifidobacterium (P = 0.026), and Enterococcus (P < 0.001) in comparison to the DM cohort. DM infants had significantly higher abundance of Staphylococcus (P = 0.014). DM, donor human milk; MOM, mother's own milk; PCoA, principal coordinates analysis.

Abundance in Group 1: increased abundance in MOM (Mother's own milk)

NCBI Quality ControlLinks
Actinomycetota
Bacteroides
Bifidobacterium
Enterococcus

Revision editor(s): Uyokeeswaran, Peace Sandy

Signature 2

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-21

Curated date: 2022/06/29

Curator: Uyokeeswaran

Revision editor(s): Uyokeeswaran, Peace Sandy

Source: Figure 2C and Figure 2D

Description: (C) When comparing longitudinal changes across samples from all study subjects, increasing relative abundance of Proteobacteria was observed. There were no significant differences observed at the phylum level during the first 2 wk of life. By week 4, microbiota from the MOM cohort had significantly higher abundance of Actinobacteria (P = 0.032) and decreased abundance of Firmicutes (P = 0.011). (D) By week 4, microbiota from the MOM cohort had significantly increased abundance of Bacteroides (P = 0.046), Bifidobacterium (P = 0.026), and Enterococcus (P < 0.001) in comparison to the DM cohort. DM infants had significantly higher abundance of Staphylococcus (P = 0.014). DM, donor human milk; MOM, mother's own milk; PCoA, principal coordinates analysis.

Abundance in Group 1: decreased abundance in MOM (Mother's own milk)

NCBI Quality ControlLinks
Bacillota
Staphylococcus

Revision editor(s): Uyokeeswaran, Peace Sandy