Disorders of Gut Microbiota and Plasma Metabolic Profiles May Be Associated with Lymph Node Tuberculosis

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

10.3390/microorganisms13071456

DOI Digital object identifier for electronic documents.

10.3390/microorganisms13071456

URI Uniform resource identifier for web resources.

Authors

Guobao Li, Haijiang Wang, Liang Yang, Shasha Zheng, Shimeng Bai, Wenying Gao, Xue Ke, Xue Li, Yun Long, Zhe Liu, Yunyan Tang

Journal

Microorganisms

Year

2025

Pages:

First page:

Keywords:

gut microbiota, lymph node tuberculosis, short-chain fatty acid

The association of gut microbiota with lymph node tuberculosis (LNTB) remains unexplored. This study employed metagenomic sequencing and plasma metabolomics analyses to investigate the role of gut microbiota in LNTB patients. Significant alterations in gut microbial diversity were observed in LNTB patients, characterized by a notable reduction in bacterial taxa involved in short-chain fatty acid (SCFA) synthesis, including Ruminococcus, Faecalibacterium, Roseburia, and Blautia, compared to healthy individuals. KEGG pathway analysis further revealed that gut dysbiosis could negatively impact SCFA biosynthesis and metabolism. Plasma metabolomics demonstrated disruptions in metabolites associated with SCFA synthesis and inflammation pathways in the LNTB group. Integrated analysis indicated significant correlations between specific gut microbiota (Blautia, Butyricicoccus, Coprococcus, Ruminococcus, Bacteroides, Clostridium) and plasma metabolites, including α-benzylbutyric acid, acetic acid, and succinic acid. Our findings demonstrate that gut microbiota dysbiosis and related metabolic dysfunction significantly reduce SCFA production in LNTB patients, potentially identifying novel therapeutic targets for LNTB management.

Experiment 1

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Curated date: 2025/07/27

Curator: Nuerteye

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Extrapulmonary tuberculosis Extrapulmonary tuberculosis,extrapulmonary tuberculosis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy volunteers

Group 1 name Corresponds to the case (exposed) group for case-control studies: Lymph node tuberculosis (LNTB) patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: All Lymph node tuberculosis (LNTB) patients cases included in this study were newly diagnosed

Group 0 sample size Number of subjects in the control (unexposed) group: 8

Group 1 sample size Number of subjects in the case (exposed) group: 9

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): prior use of therapeutic medications (including antibiotics)

Lab analysis

Sequencing type: WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: decreased

Signature 1

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Curated date: 2025/07/27

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Source: Figure 3A-B

Description: Gut microbiota characteristics in patients with lymph node tuberculosis. (A) The phylogenetic tree generated by LEfSe analysis showing the distribution of microbial taxa from the phylum to genus level between the LNTB group and the control group. (B) The histogram of LDA scores identifying significantly different bacterial taxa between patients and controls (LDA score > 2.0).

Abundance in Group 1: increased abundance in

NCBI	Quality Control	Links
Bacteroidaceae
Bacteroidales
Bacteroides
Bacteroidia
Bacteroidota
Parabacteroides
Phocaeicola
Tannerellaceae
Coprobacillaceae
Phocaeicola vulgatus
Bacteroides caccae
Bacteroides ovatus
Bacteroides thetaiotaomicron
unclassified Thomasclavelia
Parabacteroides merdae
Bacteroides xylanisolvens

Revision editor(s): Nuerteye

Signature 2

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Curated date: 2025/07/27

Curator: Nuerteye

Revision editor(s): Nuerteye

Source: Figure 3A-B

Description: Gut microbiota characteristics in patients with lymph node tuberculosis. (A) The phylogenetic tree generated by LEfSe analysis showing the distribution of microbial taxa from the phylum to genus level between the LNTB group and the control group. (B) The histogram of LDA scores identifying significantly different bacterial taxa between patients and controls (LDA score > 2.0).

Abundance in Group 1: decreased abundance in

NCBI	Quality Control	Links
Anaerobutyricum
Anaerobutyricum hallii
Blautia wexlerae
Dorea longicatena
Megasphaera
Roseburia intestinalis
Roseburia inulinivorans
Holdemanella biformis
Caudoviricetes
Uroviricota
Coprococcus
Holdemanella
Eubacteriaceae
Eubacterium
Oscillospiraceae bacterium
Bifidobacterium adolescentis
Megamonas
Selenomonadaceae
Eubacterium rectaleEubacterium rectale
Faecalibacterium prausnitzii
Dorea
Clostridium
Clostridiaceae
Collinsella
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Blautia
Roseburia
Ruminococcus
Faecalibacterium
Actinomycetota
unclassified Oscillospiraceae
Lachnospiraceae
Eubacteriales
Clostridia
Bacillota

Revision editor(s): Nuerteye