Transfer of intestinal microbiota from lean donors increases insulin sensitivity in individuals with metabolic syndrome

Study information

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study design

randomized controlled trial

Citation

PMID PubMed identifier for scientific articles.

22728514

DOI Digital object identifier for electronic documents.

10.1053/j.gastro.2012.06.031

URI Uniform resource identifier for web resources.

Authors

Vrieze A., Van Nood E., Holleman F., Salojärvi J., Kootte R.S., Bartelsman J.F., Dallinga-Thie G.M., Ackermans M.T., Serlie M.J., Oozeer R., Derrien M., Druesne A., Van Hylckama Vlieg J.E., Bloks V.W., Groen A.K., Heilig H.G., Zoetendal E.G., Stroes E.S., de Vos W.M., Hoekstra J.B., Nieuwdorp M.

Journal

Gastroenterology

Year

2012

Alterations in intestinal microbiota are associated with obesity and insulin resistance. We studied the effects of infusing intestinal microbiota from lean donors to male recipients with metabolic syndrome on the recipients' microbiota composition and glucose metabolism. Subjects were assigned randomly to groups that were given small intestinal infusions of allogenic or autologous microbiota. Six weeks after infusion of microbiota from lean donors, insulin sensitivity of recipients increased (median rate of glucose disappearance changed from 26.2 to 45.3 μmol/kg/min; P < .05) along with levels of butyrate-producing intestinal microbiota. Intestinal microbiota might be developed as therapeutic agents to increase insulin sensitivity in humans; www.trialregister.nl; registered at the Dutch Trial Register (NTR1776).

Experiment 1

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Curated date: 2025/02/04

Curator: Aleru Divine

Revision editor(s): Aleru Divine, WikiWorks

Subjects

Location of subjects: Netherlands

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Response to transplant Response to transplant,response to transplant

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Allogenic (Baseline samples)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Allogenic (6 weeks samples)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Fecal samples collected from the participants 6 weeks after allogenic lean donor gut microbiota treatment.

Group 0 sample size Number of subjects in the control (unexposed) group: 9

Group 1 sample size Number of subjects in the case (exposed) group: 9

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Human Intestinal Tract Chip

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mixed-Effects Regression

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Signature 1

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Source: Supplementary Table 3

Description: Significant changes in 16 fecal gut microbiota within the allogenic lean donor gut microbiota treatment group after 6 weeks

Abundance in Group 1: increased abundance in Allogenic (6 weeks samples)

NCBI	Quality Control	Links
Dorea formicigenerans
Mediterraneibacter gnavus
Lacrimispora sphenoides
[Clostridium] symbiosum
Coprobacillus cateniformis
Thomasclavelia ramosa
Aneurinibacillus
[Ruminococcus] lactaris
[Clostridium] nexile
Anaerotruncus colihominis
[Eubacterium] siraeum
Sporobacter termitidis
Oxalobacter formigenes
Ruminococcus callidus
Ruminococcus bromii
Ruminococcus intestinalis

Revision editor(s): Aleru Divine, WikiWorks

Experiment 3

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Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Autologous (6 weeks samples)

Lab analysis

Statistical Analysis

Signature 1

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Source: Supplementary Table 3

Description: Differences in gut microbiota identified between allogenic and autologous treatment groups.

Abundance in Group 1: increased abundance in Allogenic (6 weeks samples)

NCBI	Quality Control	Links
Dorea formicigenerans
Lacrimispora sphenoides
Coprobacillus cateniformis
[Ruminococcus] lactaris
[Clostridium] nexile
Oxalobacter formigenes

Revision editor(s): Aleru Divine, WikiWorks

Experiment 4

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Curated date: 2025/02/04

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Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Small intestine Anterior intestine,Intestinum tenue,Mid intestine,Small bowel,Small intestine,small intestine

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Small intestinal biopsy samples collected from the participants 6 weeks after allogenic lean donor gut microbiota treatment.