Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Atrayees on 2023-8-18
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Intestine Bowel,Intestinal tract,Intestine,intestine
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Colorectal cancer cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine,Colorectal cancer
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy volunteers (swc)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CRC patients (swp)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 37-88 years of age, diagnosed with colorectal cancer (swp = gut swab)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 34
- Group 1 sample size Number of subjects in the case (exposed) group
- 32
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Sanger
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-8-18
Source: Figure 4A
Description: Relative abundance of significantly different genera between CRC patients and healthy controls. (A) Genera different between swp and swc.
Abundance in Group 1: decreased abundance in CRC patients (swp)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerostipes | ||
| Catenibacterium | ||
| Faecalibacterium | ||
| Blautia | ||
| Gardnerella | ||
| Bifidobacterium | ||
| Lachnospira |
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-8-18
Source: Figure 4A
Description: Relative abundance of significantly different genera between CRC patients and healthy controls. (A) Genera different between swp and swc.
Abundance in Group 1: increased abundance in CRC patients (swp)
| NCBI | Quality Control | Links |
|---|---|---|
| Catonella | ||
| Fusobacterium | ||
| Gemella | ||
| Klebsiella | ||
| Mogibacterium | ||
| Peptostreptococcus | ||
| Porphyromonas | ||
| Selenomonas | ||
| Filifactor |
