Human Oral Buccal Microbiomes Are Associated with Farmworker Status and Azinphos-Methyl Agricultural Pesticide Exposure/Experiment 1

EditHistoryDelete

Flag for review

Your review change was submittedDuplicate this ExperimentAdd a new Signature

Reviewed Marked as Reviewed by Atrayees on 2023-7-6

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Atrayees

Subjects

Location of subjects

United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Buccal mucosa

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Air pollution air pollution,Air pollution

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Winter individuals

Group 1 name Corresponds to the case (exposed) group for case-control studies

Spring/Summer individuals

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals for whom sequencing was performed had previous spring/summer blood azinphos-methyl detection by mass spectrometry as evidence of pesticide exposure.

Group 0 sample size Number of subjects in the control (unexposed) group

82

Group 1 sample size Number of subjects in the case (exposed) group

101

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V5-V6

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Ion Torrent

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

centered log-ratio

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.01

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Richness Number of species

unchanged

Signature 1

EditHistoryDelete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2021/01/10

Curator: Victoria Goulbourne

Revision editor(s): WikiWorks, Lwaldron, Atrayees, Aiyshaaaa

Source: Table 1

Description: Wilcoxon's rank sum test of azinphos-methyl blood detection groups for compositional pertubation

Abundance in Group 1: decreased abundance in Spring/Summer individuals

NCBI	Quality Control	Links
Actinomycetales
Gemella
Granulicatella
Haemophilus
Halomonas
Micrococcales
Streptococcus

Revision editor(s): WikiWorks, Lwaldron, Atrayees, Aiyshaaaa