Gut Microbiota Composition and Fecal Metabolic Phenotype in Patients With Acute Anterior Uveitis
From BugSigDB
Jump to:navigation, search
Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Huang X, Ye Z, Cao Q, Su G, Wang Q, Deng J, Zhou C, Kijlstra A, Yang P
Journal
Investigative ophthalmology & visual science
Year
2018
PURPOSE: To investigate gut microbiota composition and fecal metabolic phenotype in patients with acute anterior uveitis. METHODS: Fecal DNA was extracted from 78 fecal samples (38 acute anterior uveitis (AAU) patients and 40 family members of patients or sex- and age-matched healthy controls) and then sequenced by high-throughput 16S rDNA analysis. Gas chromatographic mass spectrometry (GC-MS) based metabolomics was performed on 60 fecal samples (30 AAU patients and 30 healthy controls). RESULTS: A significant difference was observed in beta diversity between AAU patients and healthy controls. Eight genera including Roseburia were reduced in AAU patients, and Veillonella was increased in AAU patients as compared with healthy controls. Significance was however lost after false discovery rate (FDR) correction. The expression of seven fecal metabolites including 6-deoxy-D-glucose 1, linoleic acid, N-Acetyl-beta-D-mannosamine 3, shikimic acid, azelaic acid, isomaltose 1 and palmitoleic acid was increased in AAU patients. Linoleic acid showed a significant correlation with Roseburia and Veillonella according to Spearman correlation analysis. CONCLUSIONS: Our results did not reveal a difference in gut microbiota composition, but did show that the fecal metabolic phenotype in AAU patients was significantly different from healthy controls.
Experiment 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Anterior uveitis anterior uveitis,anterior uveitis (disease),Anterior uveitis [Ambiguous],Anterior uveitis, NOS,iridocyclitis,Uveitis, Anterior,Anterior uveitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- N (Healthy Controls)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- AAU (Acute Anterior Uveitis Patients)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients diagnosed with Acute Anterior Uveitis (AAU)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 40
- Group 1 sample size Number of subjects in the case (exposed) group
- 38
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex, ethnic group
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Figure 2, 3
Description: Taxa difference between AAU patients and healthy controls
Abundance in Group 1: increased abundance in AAU (Acute Anterior Uveitis Patients)
Revision editor(s): Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Figure 2, 3
Description: Taxa difference between AAU patients and healthy controls
Abundance in Group 1: decreased abundance in AAU (Acute Anterior Uveitis Patients)
Revision editor(s): Anne-mariesharp
Experiment 2
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Differences from previous experiment shown
Subjects
Lab analysis
Statistical Analysis
- Statistical test
- Kruskall-Wallis
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- Not specified
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Table S1
Description: List of significantly different gut microbiota between AAU patients and healthy controls
Abundance in Group 1: increased abundance in AAU (Acute Anterior Uveitis Patients)
| NCBI | Quality Control | Links |
|---|---|---|
| Negativicutes | ||
| Selenomonadales | ||
| Veillonella |
Revision editor(s): Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Table S1
Description: List of significantly different gut microbiota between AAU patients and healthy controls
Abundance in Group 1: decreased abundance in AAU (Acute Anterior Uveitis Patients)
| NCBI | Quality Control | Links |
|---|---|---|
| Peptostreptococcaceae | ||
| Lachnospiraceae | ||
| Clostridiaceae1Clostridiaceae1 | ||
| Roseburia | ||
| Lachnospiraceae incertae sedis | ||
| Dorea | ||
| Blautia | ||
| ClostridiumXIClostridiumXI | ||
| Clostridium | ||
| Odoribacter |
Revision editor(s): Anne-mariesharp
Experiment 3
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Anterior uveitis , Ankylosing spondylitis anterior uveitis,anterior uveitis (disease),Anterior uveitis [Ambiguous],Anterior uveitis, NOS,iridocyclitis,Uveitis, Anterior,Anterior uveitis,Ankylosing Spondylarthritides,Ankylosing Spondylarthritis,ankylosing spondylitis,Ankylosing Spondyloarthritides,Ankylosing Spondyloarthritis,BECHTEREW DIS,Bechterew Disease,Bechterew's Disease,BECHTEREWS DIS,Bechterews Disease,Bekhterev syndrome,Bekhterev's disease,MARIE STRUEMPELL DIS,Marie Struempell Disease,Marie-Struempell Disease,Marie-Strumpell disease,Rheumatoid Spondylitis,Spondylarthritides, Ankylosing,Spondylarthritis Ankylopoietica,Spondylarthritis, Ankylosing,Spondylitis, Ankylosing,Spondylitis, Rheumatoid,Spondyloarthritides, Ankylosing,Spondyloarthritis, Ankylosing,Ankylosing spondylitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- AAU+ AS+ (Acute Anterior Uveitis Patients with Ankylosing Spondylitis)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients diagnosed with Acute Anterior Uveitis (AAU) and Ankylosing Spondylitis (AS)
- Group 1 sample size Number of subjects in the case (exposed) group
- 17
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Table S2
Description: List of significantly different gut microbiota between AAU+ AS+ patients and healthy controls
Abundance in Group 1: increased abundance in AAU+ AS+ (Acute Anterior Uveitis Patients with Ankylosing Spondylitis)
| NCBI | Quality Control | Links |
|---|---|---|
| Veillonella |
Revision editor(s): Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Table S2
Description: List of significantly different gut microbiota between AAU+ AS+ patients and healthy controls
Abundance in Group 1: decreased abundance in AAU+ AS+ (Acute Anterior Uveitis Patients with Ankylosing Spondylitis)
| NCBI | Quality Control | Links |
|---|---|---|
| Odoribacter | ||
| Dorea |
Revision editor(s): Anne-mariesharp
Experiment 4
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Anterior uveitis anterior uveitis,anterior uveitis (disease),Anterior uveitis [Ambiguous],Anterior uveitis, NOS,iridocyclitis,Uveitis, Anterior,Anterior uveitis
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- AAU+ AS- (Acute Anterior Uveitis Patients without Ankylosing Spondylitis)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients diagnosed with Acute Anterior Uveitis (AAU) and who do not have Ankylosing Spondylitis (AS)
- Group 1 sample size Number of subjects in the case (exposed) group
- 21
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Table S3
Description: List of significantly different gut microbiota between AAU+ AS- patients and healthy controls
Abundance in Group 1: decreased abundance in AAU+ AS- (Acute Anterior Uveitis Patients without Ankylosing Spondylitis)
| NCBI | Quality Control | Links |
|---|---|---|
| ClostridiumXIClostridiumXI | ||
| Roseburia | ||
| Clostridium | ||
| Lachnospiraceae incertae sedis | ||
| Blautia | ||
| Barnesiella |
Revision editor(s): Anne-mariesharp
Experiment 5
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- HLA-B27- (Human Leukocyte Antigen B27 negative) patients
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- HLA-B27+ (Human Leukocyte Antigen B27 positive) patients
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Acute Anterior Uveitis patients that tested positive for Human Leukocyte Antigen B27 (HLA-B27)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 28
Lab analysis
Statistical Analysis
- Matched on Factors on which subjects have been matched on in a case-control study
- Not specified
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-8-12
Source: Table S4
Description: List of significantly different gut microbiota between HLA-B27+ patients and HLA-B27- patients
Abundance in Group 1: decreased abundance in HLA-B27+ (Human Leukocyte Antigen B27 positive) patients
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Blautia | ||
| Collinsella |
Revision editor(s): Anne-mariesharp
Retrieved from "https://bugsigdb.org/w/index.php?title=29625474&oldid=207745"
