Gut Microbiota is Altered in Patients with Alzheimer's Disease

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-4-3

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

29758946

DOI Digital object identifier for electronic documents.

http://dx.doi. 438 org/10.3233/JAD-180176

URI

Authors

Zhuang ZQ, Shen LL, Li WW, Fu X, Zeng F, Gui L, Lü Y, Cai M, Zhu C, Tan YL, Zheng P, Li HY, Zhu J, Zhou HD, Bu XL, Wang YJ

Journal

Journal of Alzheimer's disease : JAD

Year

2018

Keywords:

16S ribosomal RNA sequencing, Alzheimer’s disease, amyloid-β peptide, gut microbiota

Previous studies suggest that gut microbiota is associated with neuropsychiatric disorders, such as Parkinson's disease, amyotrophic lateral sclerosis, and depression. However, whether the composition and diversity of gut microbiota is altered in patients with Alzheimer's disease (AD) remains largely unknown. In the present study, we collected fecal samples from 43 AD patients and 43 age- and gender-matched cognitively normal controls. 16S ribosomal RNA sequencing technique was used to analyze the microbiota composition in feces. The composition of gut microbiota was different between the two groups. Several bacteria taxa in AD patients were different from those in controls at taxonomic levels, such as Bacteroides, Actinobacteria, Ruminococcus, Lachnospiraceae, and Selenomonadales. Our findings suggest that gut microbiota is altered in AD patients and may be involved in the pathogenesis of AD.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-4-3

Curated date: 2024/03/26

Curator: Temitayo

Revision editor(s): Temitayo, Peace Sandy, WikiWorks

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Alzheimer's disease

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with active Alzheimer's disease

Group 0 sample size Number of subjects in the control (unexposed) group: 43

Group 1 sample size Number of subjects in the case (exposed) group: 43

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 Months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.01

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-4-3

Curated date: 2024/03/26

Curator: Temitayo

Revision editor(s): Temitayo, Peace Sandy, WikiWorks

Source: Figure 4 B

Description: Differences of bacterial taxa between AD and control group b) Histogram of the LDA scores for different abundant genera. Positive LDA scores indicate the enrichment of taxa in AD group (red) relative to control group (green), and negative LDA scores indicate the depletion of taxa in AD group relative to control group. Box shows statistically significant different bacteria

Abundance in Group 1: increased abundance in Alzheimer's disease

NCBI	Quality Control	Links
Actinomycetota
Anaerotruncus
Christensenellaceae
Coprococcus
Coprococcus eutactus
Ezakiella coagulans
Gordonibacter pamelaeae
Mediterraneibacter gnavus
Oscillospiraceae
Porphyromonas bennonis
Prevotella
Rhodospirillaceae
Rhodospirillales
Sarcina
Subdoligranulum
unclassified Subdoligranulum
uncultured Bacteroides sp.

Revision editor(s): Temitayo, Peace Sandy, WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-4-3

Curated date: 2024/03/26

Curator: Temitayo

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Source: Figure 4 B

Description: Differences of bacterial taxa between AD and control group b) Histogram of the LDA scores for different abundant genera. Positive LDA scores indicate the enrichment of taxa in AD group (red) relative to control group (green), and negative LDA scores indicate the depletion of taxa in AD group relative to control group. Box shows statistically significant different bacteria

Abundance in Group 1: decreased abundance in Alzheimer's disease

NCBI	Quality Control	Links
Anaerostipes caccae
Bacteroidaceae
Bacteroides
Bacteroides acidifaciens
Blautia
Deinococcales
Deinococcus
Flavonifractor
Flavonifractor plautii
Fusobacterium varium
Lachnoclostridium
Lachnospiraceae
Lachnospiraceae bacterium
Lachnospiraceae bacterium 10-1
Negativicutes
Parabacteroides distasonis
Phocaeicola plebeius
Selenomonadales
Slackia
Stomatobaculum
Thomasclavelia ramosa
Vibrio
Vibrionaceae
Vibrionales
unclassified Ruminococcus
uncultured bacterium
Deinococcus grandis
Parasutterella secunda

Revision editor(s): Temitayo, Peace Sandy, WikiWorks