Differences in gut microbiota associated with age, sex, and stool consistency in healthy Japanese subjects/Experiment 4

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-5-7

Curated date: 2025/04/11

Curator: Montana-D

Revision editor(s): Montana-D, Anne-mariesharp

Subjects

Location of subjects
Japan
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Abnormal stool composition Abnormal faeces composition,Abnormal feces composition,Abnormal stool composition,abnormal stool composition
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Intermediate (stool in Males)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Hard (stool in Males)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Stool consistency (in males) classified as Hard using the Bristol stool scale (BSS)
Group 0 sample size Number of subjects in the control (unexposed) group
86
Group 1 sample size Number of subjects in the case (exposed) group
9
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-5-7

Curated date: 2025/04/11

Curator: Montana-D

Revision editor(s): Montana-D, Anne-mariesharp

Source: Figure 8A

Description: Significantly different genera of microbial communities among each stool consistency group for males according to their BSS (Bristol Scale Score)

Abundance in Group 1: increased abundance in Hard (stool in Males)

NCBI Quality ControlLinks
Oscillospira

Revision editor(s): Montana-D, Anne-mariesharp