Non-isoflavones Diet Incurred Metabolic Modifications Induced by Constipation in Rats via Targeting Gut Microbiota

Study information

Reviewed Marked as Reviewed by KateRasheed on 2025-6-4

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

30564225

DOI Digital object identifier for electronic documents.

10.3389/fmicb.2018.03002

URI Uniform resource identifier for web resources.

Authors

Liu J, Chang R, Zhang X, Wang Z, Wen J, Zhou T

Journal

Frontiers in microbiology

Year

2018

Keywords:

constipation, intestinal microbiota, isoflavones, metabolism, β-glucosidase

Isoflavones, presenting in leguminous plants and the normal chow diet, are known to alter intestinal microbiota, yet their deficiency has not been widely studied for its effect on constipation in biochemical state of rats. Our previous study discovered the differences in pharmacokinetic traits of isoflavones from Semen sojae praeparatum fed with normal chow diet (ISO) and non-isoflavones diet (NISO). To gain insight into the key role of intestinal microbiota in constipation and metabolic differences caused by isoflavones deficiency, we observed a significant decrease in fecal pellet numbers, fecal water content, intestinal transit rate together with the serum concentrations of substance P (SP) and vasoactive intestinal peptide (VIP) in NISO group, compared with those in the ISO group. Following 16S rRNA compositional sequencing, results excluded the changes in intestinal microbiota over time and highlighted that a total of 5 phyla and 21 genera changed significantly, among which Firmicutes, Bacteroidetes, Blautia, Prevotella, Lactobacillus and Bifidobacterium were closely related to constipation. In addition, Lactobacillus, produceing β-glucosidase which contribute to biotransform glycosides into aglycons and exert the bioactivities consequently, was decreased after non-isoflavones diet intake. Meanwhile, predicted metagenomics indicated that the pathway of glycan biosynthesis and metabolism was markedly down-regulated after non-isoflavones diet intake. Taken together, the findings suggested that the changes in the dietary components could alter the biochemical state of rats, which may be triggered by the abnormal modifications facilitated by β-glucosidase-producing bacteria. Our study shed a new strategy to explore the relationship among disease phenotypes (D), intestinal microbiota (I), enzymes (E) and traits of metabolism (T) named as "DIET," which can provide a reference for further study of the mechanism in regulation of intestinal bacteria-mediated diet on diseases.

Experiment 1

Edit History Delete

Flag for review

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by KateRasheed on 2025-6-4

Curated date: 2025/05/05

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Rattus norvegicus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Response to diet Response to diet,response to diet

Group 0 name Corresponds to the control (unexposed) group for case-control studies: CNISO (Control Non-Isoflavones)

Group 1 name Corresponds to the case (exposed) group for case-control studies: NISO (Non-Isoflavones)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Rats of experimental group that were fed with non-isoflavones diet obtained from Trophic Animal Feed High-tech Co., Ltd. (Nantong, China) for 1 week

Group 0 sample size Number of subjects in the control (unexposed) group: 6

Group 1 sample size Number of subjects in the case (exposed) group: 6

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Welch's T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: decreased

Signature 1

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by KateRasheed on 2025-6-4

Curated date: 2025/05/06

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 4A, 5A

Description: Differential abundance of intestinal microbiota between CNISO AND NISO groups at phylum and genus levels

Abundance in Group 1: increased abundance in NISO (Non-Isoflavones)

NCBI	Quality Control	Links
Blautia
Butyricimonas
Collinsella
Desulfovibrio
Gemella
Paraprevotella
Phascolarctobacterium
Roseburia

Revision editor(s): Anne-mariesharp

Signature 2

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by KateRasheed on 2025-6-4

Curated date: 2025/05/06

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 4A, 5A

Description: Differential abundance of intestinal microbiota between CNISO AND NISO groups at phylum and genus levels

Abundance in Group 1: decreased abundance in NISO (Non-Isoflavones)

NCBI	Quality Control	Links
Acetatifactor
Adlercreutzia
Alistipes
Alloprevotella
Anaeroplasma
Barnesiella
Bifidobacterium
Candidatus Saccharimonadota
Candidatus Saccharimonadota incertae sedis
Cyanobacteriota
Intestinimonas
Lactobacillus
Mycoplasmatota
Odoribacter
Prevotella
Streptophyta
Veillonella
unidentified

Revision editor(s): Anne-mariesharp