The Effect of Psyllium Husk on Intestinal Microbiota in Constipated Patients and Healthy Controls
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Jalanka J, Major G, Murray K, Singh G, Nowak A, Kurtz C, Silos-Santiago I, Johnston JM, de Vos WM, Spiller R
Journal
International journal of molecular sciences
Year
2019
Keywords:
constipation, ispaghula, microbiome, prebiotics, transit
Psyllium is a widely used treatment for constipation. It traps water in the intestine increasing stool water, easing defaecation and altering the colonic environment. We aimed to assess the impact of psyllium on faecal microbiota, whose key role in gut physiology is being increasingly recognised. We performed two randomised, placebo-controlled, double-blinded trials comparing 7 days of psyllium with a placebo (maltodextrin) in 8 healthy volunteers and 16 constipated patients respectively. We measured the patients' gastrointestnal (GI) transit, faecal water content, short-chain fatty acid (SCFA) and the stool microbiota composition. While psyllium supplement had a small but significant effect on the microbial composition of healthy adults (increasing Veillonella and decreasing Subdoligranulum), in constipated subjects there were greater effects on the microbial composition (increased Lachnospira, Faecalibacterium, Phascolarctobacterium, Veillonella and Sutterella and decreased uncultured Coriobacteria and Christensenella) and alterations in the levels of acetate and propionate. We found several taxa to be associated with altered GI transit, SCFAs and faecal water content in these patients. Significant increases in three genera known to produce butyrate, Lachnospira, Roseburia and Faecalibacterium, correlated with increased faecal water. In summary, psyllium supplementation increased stool water and this was associated with significant changes in microbiota, most marked in constipated patients.
Experiment 1
Reviewed Marked as Reviewed by Folakunmi on 2025-3-25
Subjects
- Location of subjects
- United Kingdom
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy participants at Baseline
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Healthy participants following high dose of Psyllium supplementation
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants were given 7-14g of Psyllium per day for 7days
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 Month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4-V5
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- Negative Binomial Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2025-3-24
Source: Figure 2
Description: Taxonomic differences of fecal microbiota in Healthy Adults
Abundance in Group 1: increased abundance in Healthy participants following high dose of Psyllium supplementation
| NCBI | Quality Control | Links |
|---|---|---|
| Veillonella |
Revision editor(s): Ese
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2025-3-24
Source: Figure 2
Description: Taxonomic differences of fecal microbiota in Healthy Adults
Abundance in Group 1: decreased abundance in Healthy participants following high dose of Psyllium supplementation
| NCBI | Quality Control | Links |
|---|---|---|
| Subdoligranulum |
Revision editor(s): Ese
Experiment 2
Reviewed Marked as Reviewed by Folakunmi on 2025-3-25
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Constipated Patients at baseline
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Constipated participants following Psyllium supplementation
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Constipated participants were given 21g of Psyllium per day for 7days
- Group 0 sample size Number of subjects in the control (unexposed) group
- 16
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2025-3-24
Source: Table 3
Description: Taxonomic differences of fecal microbiota in constipated participants
Abundance in Group 1: increased abundance in Constipated participants following Psyllium supplementation
| NCBI | Quality Control | Links |
|---|---|---|
| Faecalibacterium | ||
| Lachnospira | ||
| Phascolarctobacterium | ||
| Sutterella | ||
| Veillonella |
Revision editor(s): Ese
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2025-3-24
Source: Table 3
Description: Taxonomic differences of fecal microbiota in constipated participants
Abundance in Group 1: decreased abundance in Constipated participants following Psyllium supplementation
| NCBI | Quality Control | Links |
|---|---|---|
| Christensenella sp. | ||
| uncultured Coriobacterium sp. |
Revision editor(s): Ese
Experiment 3
Reviewed Marked as Reviewed by Folakunmi on 2025-3-26
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy participants at Baseline
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Constipated participants at Baseline
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Differences in fecal microbiota of Healthy and Constipated participants at Baseline
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2025-3-26
Source: Table 2
Description: Taxonomic differences of fecal microbiota in Constipated patients and Healthy patients
Abundance in Group 1: increased abundance in Constipated participants at Baseline
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomyces | ||
| Adlercreutzia | ||
| Christensenella sp. | ||
| Clostridia incertae sedis | ||
| Desulfovibrio | ||
| Erysipelotrichaceae incertae sedis | ||
| Rhodococcus | ||
| uncultured Coriobacterium sp. |
Revision editor(s): Ese
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2025-3-26
Source: Table 2
Description: Taxonomic differences of fecal microbiota in Constipated patients and Healthy patients
Abundance in Group 1: decreased abundance in Constipated participants at Baseline
| NCBI | Quality Control | Links |
|---|---|---|
| Lachnospira | ||
| SuterellaSuterella |
Revision editor(s): Ese
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