Metabolic phenotypes and the gut microbiota in response to dietary resistant starch type 2 in normal-weight subjects: a randomized crossover trial

Study information

Reviewed Marked as Reviewed by KateRasheed on 2025-10-17

study design

randomized controlled trial

Citation

PMID PubMed identifier for scientific articles.

30894560

DOI Digital object identifier for electronic documents.

https://doi.org/10.1038/s41598-018-38216-9

URI Uniform resource identifier for web resources.

https://www.nature.com/articles/s41598-018-38216-9

Authors

Zhang L., Ouyang Y., Li H., Shen L., Ni Y., Fang Q., Wu G., Qian L., Xiao Y., Zhang J., Yin P., Panagiotou G., Xu G., Ye J., Jia W.

Journal

Scientific reports

Year

2019

Resistant starch (RS) has been reported to reduce body fat in obese mice. However, this effect has not been demonstrated in humans. In this study, we tested the effects of RS in 19 volunteers with normal body weights. A randomized, double-blinded and crossover design clinical trial was conducted. The study subjects were given either 40 g high amylose RS2 or energy-matched control starch with three identical diets per day throughout the study. The effect of RS was evaluated by monitoring body fat, glucose metabolism, gut hormones, gut microbiota, short-chain fatty acids (SCFAs) and metabolites. The visceral and subcutaneous fat areas were significantly reduced following RS intake. Acetate and early-phase insulin, C-peptide and glucagon-like peptide-1 (GLP-1) secretion were increased, and the low-density lipoprotein cholesterol (LDL-C) and blood urea nitrogen (BUN) levels were decreased after the RS intervention. Based on 16S rRNA sequencing, certain gut microbes were significantly decreased after RS supplementation, whereas the genus Ruminococcaceae_UCG-005 showed an increase in abundance. Other potential signatures of the RS intervention included Akkermansia, Ruminococcus_2, Victivallis, and Comamonas. Moreover, the baseline abundance of the genera Streptococcus, Ruminococcus_torques_group, Eubacterium_hallii_group, and Eubacterium_eligens_group was significantly associated with the hormonal and metabolic effects of RS. These observations suggest that a daily intake of 40 g of RS is effective in modulating body fat, SCFAs, early-phase insulin and GLP-1 secretion and the gut microbiota in normal-weight subjects.

Experiment 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-10-17

Curated date: 2025/10/09

Curator: Lindy

Revision editor(s): Lindy

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Response to diet Response to diet,response to diet

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Control starch intake (CS)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Resistant starch intake (RS)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: These are the participants that consumed 40 g/day of high-amylose maize resistant starch type 2 for 4 weeks during the intervention phase.

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: log transformation

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample: unchanged

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-10-17

Curated date: 2025/10/10

Curator: Lindy

Revision editor(s): Lindy

Source: Figure 3A

Description: Differentially abundant bacterial taxa identified after resistant starch type 2 (RS2) supplementation compared with control starch (CS) in healthy humans (adults).

Abundance in Group 1: increased abundance in Resistant starch intake (RS)

NCBI	Quality Control	Links
Tyzzerella_3Tyzzerella_3
Lachnospiraceae_ND3007_groupLachnospiraceae_ND3007_group
Ruminococcaceae bacterium UCG-005

Revision editor(s): Lindy

Signature 2

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Reviewed Marked as Reviewed by KateRasheed on 2025-10-17

Curated date: 2025/10/11

Curator: Lindy

Revision editor(s): Lindy

Source: Figure 3A

Description: Differentially abundant bacterial taxa identified were significantly decreased after resistant starch type 2 (RS2) supplementation compared with control starch (CS) in healthy humans (adults).

Abundance in Group 1: decreased abundance in Resistant starch intake (RS)

NCBI	Quality Control	Links
Anaerostipes
Bacteroides
Blautia
Dorea
Holdemanella
Lachnoclostridium
Lachnospira eligens
Paraprevotella
Phascolarctobacterium
Ruminiclostridium_6Ruminiclostridium_6
Lachnospiraceae_UCG-010Lachnospiraceae_UCG-010
Erysipelotrichaceae_UCG-003Erysipelotrichaceae_UCG-003
Coprococcus_3Coprococcus_3
Ruminococcaceae_UCG-002Ruminococcaceae_UCG-002
Coprococcus_1Coprococcus_1
Lachnospiraceae_UCG-004Lachnospiraceae_UCG-004

Revision editor(s): Lindy