Meta-analysis of fecal metagenomes reveals global microbial signatures that are specific for colorectal cancer/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Lwaldron on 2021/06/26
Subjects
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Colorectal carcinoma cancer of large bowel,cancer of large intestine,cancer of the large bowel,cancer of the large intestine,carcinoma of colorectum,carcinoma of large bowel,carcinoma of large intestine,carcinoma of the large bowel,carcinoma of the large intestine,colorectal (colon or rectal) cancer,colorectal cancer,colorectal carcinoma,colorectum carcinoma,CRC,large bowel cancer,large bowel carcinoma,large intestine cancer,large intestine carcinoma,Colorectal carcinoma
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- colorectal cancer
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Colorectal cancer cases from eight independent studies
- Group 0 sample size Number of subjects in the control (unexposed) group
- 392
- Group 1 sample size Number of subjects in the case (exposed) group
- 386
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Fig. 1 (species) and extended data fig. 4 (genera)
Description: Species and genera enriched in CRC vs controls
Abundance in Group 1: increased abundance in colorectal cancer
Signature 2
Source: Extended Data Fig. 4
Description: Genera enriched in CRC vs controls
Abundance in Group 1: increased abundance in colorectal cancer
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerococcus | ||
| Eubacterium | ||
| Fusobacterium | ||
| Gemella | ||
| Hungatella | ||
| Lachnoclostridium | ||
| Leptotrichia | ||
| Parvimonas | ||
| Peptostreptococcus | ||
| Porphyromonas | ||
| Solobacterium | ||
| Subdoligranulum |
Signature 3
Source: Fig. 1
Description: Species enriched in CRC vs controls
Abundance in Group 1: increased abundance in colorectal cancer
