Adhesive Bifidobacterium Induced Changes in Cecal Microbiome Alleviated Constipation in Mice
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control (Constipation mice without treatment)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- ANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: increased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Lactobacillus | ||
| Prevotella | ||
| unclassified Helicobacteraceae |
Signature 2
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: decreased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Anaeroplasma | ||
| Clostridium | ||
| Coprobacillus | ||
| Odoribacter | ||
| Parabacteroides | ||
| Ruminococcus | ||
| Sporobacter | ||
| Turicibacter | ||
| unclassified Lachnospiraceae | ||
| Oscillibacter |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control ( Constipation mice without treatment )
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB2(Combined multiple strains of Bifidobacterium with no adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: increased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Bifidobacterium | ||
| Lactobacillus | ||
| Odoribacter | ||
| Sporobacter |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: decreased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridium | ||
| Parabacteroides | ||
| unclassified Lachnospiraceae | ||
| Coprobacillus | ||
| Oscillibacter | ||
| Ruminococcus | ||
| Sporobacter | ||
| Turicibacter | ||
| unclassified Helicobacteraceae | ||
| Alistipes |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 3
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement , Constipation Treatment outcome measurement,treatment outcome measurement,Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal (Healthy mice)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: increased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Lactobacillus | ||
| unclassified Helicobacteraceae |
Signature 2
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: decreased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Anaeroplasma | ||
| Clostridium | ||
| Odoribacter | ||
| Oscillibacter | ||
| Ruminococcus | ||
| Sporobacter | ||
| Turicibacter | ||
| unclassified Lachnospiraceae |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 4
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB2(Combined multiple strains of Bifidobacterium with no adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: increased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Bifidobacterium | ||
| Odoribacter | ||
| unclassified Helicobacteraceae | ||
| Alistipes |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: decreased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella | ||
| unclassified Lachnospiraceae | ||
| Lactobacillus | ||
| Turicibacter | ||
| Oscillibacter | ||
| Ruminococcus | ||
| Sporobacter |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 5
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: increased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Prevotella | ||
| Lactobacillus | ||
| unclassified Helicobacteraceae |
Signature 2
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: decreased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Anaeroplasma | ||
| Clostridium | ||
| Odoribacter | ||
| Sporobacter |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 6
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal (Healthy mice)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Control (Constipation mice without treatment)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group were given 0.25 mL normal saline (NS) using intragastric administration once a day for 17 days and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: increased abundance in Control (Constipation mice without treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Anaeroplasma | ||
| Clostridium | ||
| Coprobacillus | ||
| Parabacteroides | ||
| Sporobacter | ||
| Turicibacter | ||
| unclassified Helicobacteraceae |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 3, 4 & 5
Description: Relative abundance (%) of fecal microbial taxa at the genus level in constipated mice fed CMB
Abundance in Group 1: decreased abundance in Control (Constipation mice without treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella | ||
| Lactobacillus |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 7
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control (Constipation mice without treatment)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The CMB1 group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Bifidobacterium | ||
| Clostridium | ||
| Coprococcus | ||
| Dehalobacterium | ||
| Dorea | ||
| Lactobacillus | ||
| Ruminococcus | ||
| unclassified Muribaculaceae |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Parabacteroides | ||
| unclassified F-16unclassified F-16 |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 8
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB2(Combined multiple strains of Bifidobacterium with no adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Bacteroides | ||
| Bifidobacterium | ||
| Clostridium | ||
| Lactobacillus | ||
| Oscillospira | ||
| Odoribacter | ||
| Mucispirillum |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Dehalobacterium | ||
| Desulfovibrio | ||
| Dorea | ||
| Rikenella | ||
| unclassified Helicobacteraceae | ||
| Parabacteroides | ||
| Anaeroplasma |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 9
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation , Treatment outcome measurement Constipation,Costiveness,Dyschezia,constipation,Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal (Healthy mice)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Bacteroides | ||
| Bifidobacterium | ||
| Coprococcus | ||
| Dehalobacterium | ||
| Desulfovibrio | ||
| Lactobacillus | ||
| unclassified Muribaculaceae |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillospira | ||
| Rikenella | ||
| Odoribacter | ||
| Mucispirillum | ||
| unclassified Helicobacteraceae | ||
| Akkermansia | ||
| unclassified Lachnospiraceae |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 10
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB2(Combined multiple strains of Bifidobacterium with no adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Bifidobacterium | ||
| Lactobacillus | ||
| unclassified F-16unclassified F-16 | ||
| unclassified Muribaculaceae |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Desulfovibrio | ||
| Dorea | ||
| Rikenella | ||
| unclassified Helicobacteraceae | ||
| unclassified Lachnospiraceae | ||
| Clostridium |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 11
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB2 with no adhesion properties)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Coprococcus | ||
| Dehalobacterium | ||
| Desulfovibrio | ||
| Dorea | ||
| Rikenella | ||
| Ruminococcus | ||
| unclassified Helicobacteraceae | ||
| unclassified Muribaculaceae |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Bifidobacterium | ||
| Mucispirillum | ||
| Odoribacter | ||
| Oscillospira | ||
| unclassified F-16unclassified F-16 |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 12
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal (Healthy mice)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Control (Constipation mice without treatment)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group were given 0.25 mL normal saline (NS) using intragastric administration once a day for 17 days and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in Control (Constipation mice without treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Adlercreutzia | ||
| Anaeroplasma | ||
| Dehalobacterium | ||
| Parabacteroides | ||
| unclassified Muribaculaceae | ||
| unclassified F-16unclassified F-16 |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in Control (Constipation mice without treatment)
| NCBI | Quality Control | Links |
|---|---|---|
| Dorea | ||
| Ruminococcus | ||
| Rikenella | ||
| Odoribacter | ||
| Mucispirillum | ||
| Clostridium | ||
| Oscillospira | ||
| Akkermansia | ||
| unclassified Lachnospiraceae |
Revision editor(s): Joiejoie, Anne-mariesharp
Experiment 13
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces , Caecum Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces,Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Sampling site Sampling site,sampling site
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal (Healthy mice) (fecal sample)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- C-Normal (Healthy mice) (cecal sample)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- These are healthy mice that were given 0.25 mL normal saline (NS) using intragastric administration once a day for 17 days.
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples.
Abundance in Group 1: increased abundance in C-Normal (Healthy mice) (cecal sample)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Clostridium | ||
| Mucispirillum | ||
| Oscillospira | ||
| Rikenella | ||
| Ruminococcus | ||
| unclassified Helicobacteraceae | ||
| unclassified Lachnospiraceae | ||
| Dorea |
Signature 2
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples
Abundance in Group 1: decreased abundance in C-Normal (Healthy mice) (cecal sample)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Odoribacter |
Revision editor(s): Mautin
Experiment 14
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control (Constipation mice without treatment) (fecal sample)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- C-Control (Constipation mice without treatment) (cecal sample)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group were given 0.25 mL normal saline (NS) using intragastric administration once a day for 17 days and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples.
Abundance in Group 1: increased abundance in C-Control (Constipation mice without treatment) (cecal sample)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Dorea | ||
| unclassified Helicobacteraceae | ||
| Mucispirillum | ||
| Oscillospira | ||
| Parabacteroides | ||
| Rikenella | ||
| Ruminococcus | ||
| Akkermansia |
Signature 2
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples
Abundance in Group 1: decreased abundance in C-Control (Constipation mice without treatment) (cecal sample)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Odoribacter |
Revision editor(s): Mautin
Experiment 15
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties) (fecal sample)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- C-CMB1 (Constipation mice treated with CMB1 with adhesion properties) (cecal sample)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples.
Abundance in Group 1: increased abundance in C-CMB1 (Constipation mice treated with CMB1 with adhesion properties) (cecal sample)
Signature 2
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples.
Abundance in Group 1: decreased abundance in C-CMB1 (Constipation mice treated with CMB1 with adhesion properties) (cecal sample)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Lactobacillus | ||
| Odoribacter |
Revision editor(s): Mautin
Experiment 16
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB2 with no adhesion properties) (fecal sample)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- C-CMB2 (Constipation mice treated with CMB2 with no adhesion properties) (cecal sample)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB2(Combined multiple strains of Bifidobacterium with no adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples.
Abundance in Group 1: increased abundance in C-CMB2 (Constipation mice treated with CMB2 with no adhesion properties) (cecal sample)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Anaeroplasma | ||
| Clostridium | ||
| Dorea | ||
| Mucispirillum | ||
| Oscillospira | ||
| Parabacteroides | ||
| Rikenella | ||
| Ruminococcus | ||
| unclassified Lachnospiraceae | ||
| unclassified Helicobacteraceae |
Signature 2
Source: Figure 9,10,11
Description: The genus-level comparison of fecal samples and caecum content samples.
Abundance in Group 1: decreased abundance in C-CMB2 (Constipation mice treated with CMB2 with no adhesion properties) (cecal sample)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Lactobacillus | ||
| Odoribacter |
Revision editor(s): Mautin
