Gut Microbiota Are Associated With Psychological Stress-Induced Defections in Intestinal and Blood-Brain Barriers/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-6-3

Curated date: 2025/05/06

Curator: Shulamite

Revision editor(s): Shulamite, Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Rattus norvegicus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to stress response to abiotic stress,response to biotic stress,Response to stress,response to stress
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Psychological-stress model group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Rats that received psychological stress stimulation through the communication box system for 28 days.
Group 0 sample size Number of subjects in the control (unexposed) group
6
Group 1 sample size Number of subjects in the case (exposed) group
6

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-3

Curated date: 2025/05/06

Curator: Shulamite

Revision editor(s): Shulamite

Source: Fig. 4E and F

Description: Results of LEfSe analysis and key taxa found by LDA analysis (multigroup comparison strategy: one-against-all, LDA > 2, P < 0.05).

Abundance in Group 1: increased abundance in Psychological-stress model group

NCBI Quality ControlLinks
Acidaminococcaceae
Aerococcaceae
Allisonella
Allobaculum
Anaerovoracaceae
Bacillales
Bacteroidales
Catenisphaera
Coprococcus
Coriobacteriaceae
Desulfovibrio
Erysipelotrichaceae
Erysipelotrichales
Eubacteriales Family XII. Incertae Sedis
Gemella
Globicatella
Holdemanella
Negativicutes
Odoribacter
Oribacterium
Peptococcus
Phascolarctobacterium
Prevotellaceae
Selenomonadales
Treponema
unclassified Bacteroidales
unclassified Erysipelotrichaceae
Prevotellaceae_UCG_004Prevotellaceae_UCG_004

Revision editor(s): Shulamite

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-6-3

Curated date: 2025/05/07

Curator: Shulamite

Revision editor(s): Shulamite, Victoria, KateRasheed

Source: Fig. 4E and F

Description: Results of LEfSe analysis and key taxa found by LDA analysis (multigroup comparison strategy: one-against-all, LDA > 2, P < 0.05).

Abundance in Group 1: decreased abundance in Psychological-stress model group

NCBI Quality ControlLinks
Bilophila
Butyricimonas
Christensenellaceae
Clostridium
Eubacteriales
Faecalitalea
Intestinimonas
Mycoplasma
Mycoplasmataceae
Mycoplasmatales
Oscillibacter
Peptococcaceae
Peptostreptococcaceae
Romboutsia
Turicibacter
Tyzzerella
[Bacteroides] pectinophilus
unclassified Eubacteriales
Prevotellaceae_UCG_001Prevotellaceae_UCG_001
Ruminiclostridium_6Ruminiclostridium_6
Christensenellaceae_R_7_groupChristensenellaceae_R_7_group
Ruminococcaceae_UCG_010Ruminococcaceae_UCG_010
Clostridiales_vadinBB60_groupClostridiales_vadinBB60_group
Ruminococcaceae_UCG_013Ruminococcaceae_UCG_013

Revision editor(s): Shulamite, Victoria, KateRasheed