The composition of intestinal microbiota and its association with functional constipation of the elderly patients
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Guo M, Yao J, Yang F, Liu W, Bai H, Ma J, Ma X, Zhang J, Fang Y, Miao Y, Sun J, Zhang Y, Zhao H
Journal
Future microbiology
Year
2020
Keywords:
16s rRNA gene sequencing, functional constipation, intestinal microbiota composition, the elderly
Aim: To identify intestinal microbiota compositions in elderly functional constipation (FC) patients. Materials & methods: Fecal samples from 61 FC patients and 48 healthy age-matched volunteers were analyzed through 16S rRNA gene sequencing. Results: The intestinal microbiota compositions of FC patients were significantly different from healthy controls. Additionally, the species diversity of healthy controls was greater than that of FC patients. Indeed, the abundance of Firmicutes and Proteobacteria was significantly decreased, whereas that of Bacteroides, Prevotella, Lactococcus, Ruminococcus and Butyricimonas was remarkably increased in FC patients. Conclusion: Elderly FC patients appear to have a unique intestinal microbiota profile. Our findings should provide insight regarding the pathogenic mechanism of FC and evidence for exploring new therapeutic strategies in elderly FC patients.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Chronic constipation Chronic constipation,Infrequent bowel movements,chronic constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Heathy volunteers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Functional constipation (FC) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with functional constipation (FC)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 48
- Group 1 sample size Number of subjects in the case (exposed) group
- 61
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Matched on Factors on which subjects have been matched on in a case-control study
- age
Alpha Diversity
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Source: Figure 3B
Description: The intestinal microbiota composition between functional constipation patients and healthy controls at the genus level
Abundance in Group 1: increased abundance in Functional constipation (FC) group
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Butyricimonas | ||
| Campylobacter | ||
| Candidatus Epulonipiscium | ||
| Clostridium | ||
| Rheinheimera | ||
| Sutterella |
Revision editor(s): Tosin
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Source: Figure 3B
Description: The intestinal microbiota composition between functional constipation patients and healthy controls at the genus level
Abundance in Group 1: decreased abundance in Functional constipation (FC) group
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Allobaculum | ||
| Atopobium | ||
| Dehalobacterium | ||
| Deinococcus | ||
| Edwardsiella | ||
| Escherichia | ||
| Lactococcus | ||
| Prevotella | ||
| Pseudomonas | ||
| Sphingomonas | ||
| Thermus | ||
| Veillonella |
Revision editor(s): Tosin
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Differences from previous experiment shown
Subjects
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Source: Figure 4
Description: The comparison of different intestinal microbiota species between functional constipation patients and healthy controls based on the classification level tree
Abundance in Group 1: increased abundance in Functional constipation (FC) group
Revision editor(s): Tosin
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-4-4
Source: Figure 4
Description: The comparison of different intestinal microbiota species between functional constipation patients and healthy controls based on the classification level tree
Abundance in Group 1: decreased abundance in Functional constipation (FC) group
Revision editor(s): Tosin
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