Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota/Experiment 4
From BugSigDB
Subjects
- Location of subjects
- Mali
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Sputum , Feces Expectoration,Sputum,sputum,Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Pulmonary tuberculosis lung TB,lung tuberculosis,pulmonary TB,pulmonary tuberculosis,Tuberculosis, Pulmonary,Pulmonary tuberculosis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Mycobacterium africanum plus anti tuberculosis treatment (MAF+HRZE)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mycobacterium tuberculosis plus anti tuberculosis treatment (MTB+HRZE)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- composed of newly infected active pulmonary tuberculosis patients defined by positive sputum culture who received anti tuberculosis treatment for the first two months.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 21
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- -V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Figure 3C
Description: Perturbation in the intestinal microbiome composition due to HRZE treatment in both MAF and MTB infection. c. Pairwise LEfSe comparisons were performed between the three groups to identify differentially abundant families. Taxa are filtered for p <0.05 and LDA score > 2.
Abundance in Group 1: increased abundance in Mycobacterium tuberculosis plus anti tuberculosis treatment (MTB+HRZE)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacteriaceae | ||
| Streptococcaceae | ||
| Coriobacteriaceae incertae sedis |
Revision editor(s): Nuerteye
