Characterizing the gut microbiota in females with infertility and preliminary results of a water-soluble dietary fiber intervention study

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Needs review
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Komiya S, Naito Y, Okada H, Matsuo Y, Hirota K, Takagi T, Mizushima K, Inoue R, Abe A, Morimoto Y
Journal
Journal of clinical biochemistry and nutrition
Year
2020
Keywords:
Bifidobacterium, dietary fiber, gut microbiota, infertility
Despite the advances in assisted reproductive technology, approximately 8-12% of the individuals worldwide who are willing to conceive are unable to do so. Fertility depends on a receptive state of the endometrium and hormonal adaptations as well as the immune system. Local and systemic immunities are greatly influenced by the microbiota. The aim of the present study was to compare the gut microbiota in female patients with that in infertility with fertile control subjects and to evaluate the effect of prebiotic partially hydrolyzed guar gum supplementation on gut dysbiosis and the outcome of pregnancy in patients treated with assisted reproductive technology. Dietary fiber can reconstitute the host intestinal microbiota and modify the immune function; however, clinical data regarding the effect of dietary fiber treatment on the success of assisted reproductive technology is lacking. To investigate the gut microbiota in fertile and infertile females, we conducted 16S metagenomic analysis of fecal samples. In total 18 fertile female subjects and 18 patients with infertility matched by age were recruited, and fecal samples were obtained to analyze the gut microbiome using 16S rRNA V3-V4 sequencing. The unweighted and weighted principal coordinate analyses showed a trend indicating microbial structural differences in β-diversity between these two groups. The abundance of the phylum Verrucomicrobia was higher in patients with infertility. At the genus level, a decrease in the abundance of the genera Stenotrophomonas, Streptococcus, and Roseburia and an increase in the abundance of the genera Unclassified [Barnesiellaceae] and Phascolarctobacterium was observed in patients with infertility. Twelve patients agreed to receive the combined therapy comprising embryo transfer by assisted reproductive technology and oral supplementation with partially hydrolyzed guar gum. The success of pregnancy by this combined therapy was 58.3% (7/12), and the failure was 41.7% (5/12). Predictive factors for pregnancy before treatment were characterized by a decrease in the abundance of Paraprevotella and Blautia and an increase in the abundance of Bifidobacterium. Predictive factors for pregnancy before treatment were characterized by a decrease in the abundance of Paraprevotella and Blautia and an increase tendency in the abundance of Bifidobacterium. In conclusion, the present study showed differences in the abundance of gut microbiota between fertile and infertile groups; moreover, partially hydrolyzed guar gum supplementation helped improve gut dysbiosis and the success of pregnancy in females with infertility.

Experiment 1


Needs review

Curated date: 2025/06/25

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Subjects

Location of subjects
Japan
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Female infertility female infertility,female reproductive system infertility,female reproductive system infertility disorder,female sterility,female sub-fertility,female subfertility,infertility disorder of female reproductive system,postpartum sterility,sterility, female,sterility, postpartum,sub fertility, female,sub-fertility, female,subfertility, female,Female infertility
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Control group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Infertile group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Female patients with infertility
Group 0 sample size Number of subjects in the control (unexposed) group
18
Group 1 sample size Number of subjects in the case (exposed) group
18

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Welch's T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Matched on Factors on which subjects have been matched on in a case-control study
age

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2025/06/25

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4

Description: Comparative analysis of the taxonomic composition of the microbial community at the genus level between control subjects and patients with infertility.

Abundance in Group 1: increased abundance in Infertile group

NCBI Quality ControlLinks
Acinetobacter
unclassified Alphaproteobacteria
Vulcanimicrobiota
Dysgonomonas
Anaeroplasma
Rikenella
Alistipes
Bacillus
Eggerthella
unclassified Barnesiellaceae
Anaerotruncus
Phascolarctobacterium
unclassified Anaerovoracaceae
Desulfovibrio
Coprobacillus

Revision editor(s): Aleru Divine

Signature 2

Needs review

Curated date: 2025/06/25

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 4

Description: Comparative analysis of the taxonomic composition of the microbial community at the genus level between control subjects and patients with infertility.

Abundance in Group 1: decreased abundance in Infertile group

NCBI Quality ControlLinks
Aeromonadaceae
unclassified Oxalobacteraceae
Actinobaculum
Hydrogenophaga
Leucobacter
Stenotrophomonas
Achromobacter
Ochrobactrum
unclassified Microbacteriaceae
Gemellaceae
Streptococcus
Granulicatella
Roseburia
Candidatus Epulonipiscium
Morganella

Revision editor(s): Aleru Divine

Experiment 2


Needs review

Curated date: 2025/06/25

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non-pregnant group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Pregnant group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Female patients who were pregnant after combined therapy
Group 0 sample size Number of subjects in the control (unexposed) group
5
Group 1 sample size Number of subjects in the case (exposed) group
7

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/06/25

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 6

Description: Comparative analysis of the taxonomic composition of the microbial community at the genus level between the pregnant and the non-pregnant groups after treatment with partially hydrolyzed guar gum

Abundance in Group 1: decreased abundance in Pregnant group

NCBI Quality ControlLinks
Candidatus Epulonipiscium
Blautia
Paraprevotella

Revision editor(s): Aleru Divine