Changes of vaginal microbiota during cervical carcinogenesis in women with human papillomavirus infection

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

32941440

DOI Digital object identifier for electronic documents.

10.1371/journal.pone.0238705

URI Uniform resource identifier for web resources.

Authors

So K.A., Yang E.J., Kim N.R., Hong S.R., Lee J.H., Hwang C.S., Shim S.H., Lee S.J., Kim T.J.

Journal

PloS one

Year

2020

OBJECTIVE: To evaluate the changes of vaginal microbiota during cervical carcinogenesis in women with high-risk human papillomavirus infection. MATERIALS AND METHODS: Vaginal microbiota was analyzed using next-generation sequencing in women with normal, cervical intraepithelial neoplasia (CIN), or cervical cancer. RESULTS: A marked decrease of Lactobacillus crispatus was found in the CIN/cancer groups compared with that in the normal group. The diversity of microorganisms increased in patients with CIN or cervical cancer with HPV infection. Atopobium vaginae (OR 4.33, 95% CI 1.15-16.32), Dialister invisus (OR 4.89, 95% CI 1.20-19.94), Finegoldia magna (OR 6.00, 95% CI 1.08-33.27), Gardnerella vaginalis (OR 7.43, 95% CI 1.78-31.04), Prevotella buccalis (OR 11.00, 95% CI 2.00-60.57), and Prevotella timonensis (OR 6.00, 95% CI 1.46-24.69) were significantly associated with the risk of CIN 2/3 or cervical cancer. CONCLUSION: Women with the CIN and cervical cancer showed a high diversity in vaginal microbiota. Depletion of Lactobacillus crispatus and increased abundance of anaerobic bacteria were detected in women with cervical disease.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

Curated date: 2021/03/21

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, LGeistlinger, Cynthia Anderson, Claregrieve1

Subjects

Location of subjects: South Korea

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Vaginal fluid Vaginal discharge,Vaginal secretion,Vaginal fluid,vaginal fluid

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: CIN2+/cervical cancer

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patients with cervical intraepithelial neoplasia 2+ or cervical cancer confirmed by biopsy

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 20

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Chi-Square

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

Curated date: 2021/03/21

Curator: Cynthia Anderson

Revision editor(s): WikiWorks, Cynthia Anderson, Claregrieve1

Source: Table 2

Description: Differential microbial abundance between healthy controls and patients with cervical disease with CIN 2 or 3 and cervical cancer

Abundance in Group 1: increased abundance in CIN2+/cervical cancer

NCBI	Quality Control	Links
Dialister invisus
Fannyhessea vaginae
Finegoldia magna
Gardnerella vaginalis
Hoylesella buccalis
Hoylesella timonensis

Revision editor(s): WikiWorks, Cynthia Anderson, Claregrieve1