Dysbiosis of the Saliva Microbiome in Patients With Polycystic Ovary Syndrome/Experiment 4
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-5-18
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- SalivaSaliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Polycystic ovary syndrome Cystic disease of ovaries,hyperandrogenemia,Multicystic ovaries,multicystic ovaries,Ovarian Degeneration, Sclerocystic,Ovarian Syndrome, Polycystic,Ovarian Syndromes, Polycystic,Ovaries, Sclerocystic,Ovary Syndrome, Polycystic,Ovary, Sclerocystic,PCO - Polycystic ovaries,Pco1,PCOD - Polycystic ovarian disease,PCOS,Pcos,PCOS - Polycystic ovarian syndrome,PCOS1,Polycystic ovarian disease,polycystic ovarian disease,Polycystic ovarian syndrome,Polycystic ovaries,polycystic ovaries,Polycystic ovaries (disorder),polycystic ovary,polycystic ovary syndrome,polycystic ovary syndrome 1,Sclerocystic Ovarian Degeneration,Sclerocystic Ovaries,Sclerocystic Ovary,Sclerocystic Ovary Syndrome,Stein Leventhal Syndrome,Stein-Leventhal synd.,Stein-Leventhal Syndrome,Stein-Leventhal syndrome,Syndrome, Polycystic Ovary,Syndrome, Stein-Leventhal,Polycystic ovary syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy control at Zeitgeber (ZT) 18
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- PCOS patients at Zeitgeber (ZT) 18
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Samples collected from patients with Polycystic Ovary Syndrome who were diagnosed according to the Rotterdam Criteria, who met two out of three of the following criteria: clinical/biochemical hyperandrogenism, oligo-/anovulation, and polycystic ovaries at Zeitgeber (ZT) 18
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- use of antibiotics within 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
- Matched on Factors on which subjects have been matched on in a case-control study
- age, body mass index, metabolic syndrome
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Needs review
Source: Figure 3D
Description: Identification of specific bacterial taxa in PCOS patients and controls at different time points. Linear discriminant analysis (LDA) effect size (LEfSe) was used to identify biomarkers with significant differences between the two groups: Control.ZT18 vs. PCOS.ZT18
Abundance in Group 1: increased abundance in PCOS patients at Zeitgeber (ZT) 18
Revision editor(s): Idiaru angela, WikiWorks
Signature 2
Needs review
Source: Figure 3D
Description: Identification of specific bacterial taxa in PCOS patients and controls at different time points. Linear discriminant analysis (LDA) effect size (LEfSe) was used to identify biomarkers with significant differences between the two groups: Control.ZT18 vs. PCOS.ZT18
Abundance in Group 1: decreased abundance in PCOS patients at Zeitgeber (ZT) 18
| NCBI | Quality Control | Links |
|---|---|---|
| Neisseria flava | ||
| unclassified Cyanobacteriota | ||
| Cyanobacteriota | ||
| Leptotrichiaceae | ||
| Leptotrichia | ||
| Prevotella histicola | ||
| unclassified Prevotellaceae |
Revision editor(s): Idiaru angela, WikiWorks
