Mucosa-associated gut microbiome in Japanese patients with functional constipation

Study information

Reviewed Marked as Reviewed by Svetlana up on 2025-3-19

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

33879972

DOI Digital object identifier for electronic documents.

10.3164/jcbn.20-93

URI

Authors

Sugitani Y, Inoue R, Inatomi O, Nishida A, Morishima S, Imai T, Kawahara M, Naito Y, Andoh A

Journal

Journal of clinical biochemistry and nutrition

Year

2021

Keywords:

butyrate, constipation, microbiota

The number of patients with chronic constipation is increasing in Japan. We investigated the gut mucosa-associated microbiome in Japanese patients with functional constipation. Diagnosis was made according to the Rome IV criteria. Mucosal samples were obtained by gentle brushing of mucosa surfaces. The gut microbiome was analyzed using 16S rRNA gene sequencing. There were no significant differences in bacteria α-diversity such as richness and evenness. The PCoA indicated significant structural differences between the constipation group and healthy controls (p = 0.017 for unweighted and p = 0.027 for weighted). The abundance of the phylum Bacteroidetes was significantly higher in the constipation group. The abundance of the genera Streptococcus, Fusobacterium, Comamonas, and Alistipes was significantly higher in the constipation group. The abundance of the genera Acinetobacter, Oscillospilla, Mucispirillum, Propinibacterium, and Anaerotruncus was significantly lower in the constipation group. In the constipation group, the proportion of genes responsible for sulfur metabolism, selenocompound metabolism, sulfur relay system was significantly higher and the proportion of d-arginine and d-ornithine metabolism and flavonoid biosynthesis was significantly lower. In conclusion, we identified differences of the mucosa-associated microbiome between Japanese patients with functional constipation and healthy controls. The mucosa-associated microbiome of functional constipation was characterized by higher levels of Bacteroidetes (Alistipes).

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2025-3-19

Curated date: 2025/03/17

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects: Japan

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Mucus Mucus,mucus

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Chronic constipation Chronic constipation,Infrequent bowel movements,chronic constipation

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Patients with Functional constipation (FC)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Male patients diagnosed with functional constipation according to the Rome IV criteria with average age of 71.4 years (37–84)

Group 0 sample size Number of subjects in the control (unexposed) group: 14

Group 1 sample size Number of subjects in the case (exposed) group: 15

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Kruskall-Wallis

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Matched on Factors on which subjects have been matched on in a case-control study: age

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Richness Number of species: unchanged

Signature 1

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Source: Figure 2

Description: Comparative analyses of the taxonomic composition of the microbial community at the phylum level

Abundance in Group 1: increased abundance in Patients with Functional constipation (FC)

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Bacteroidota

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Experiment 2

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Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Statistical test: LEfSe

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Richness Number of species: unchanged

Signature 1

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Source: Figure 3

Description: Differential abundant taxa between the constipation group and healthy controls

Abundance in Group 1: increased abundance in Patients with Functional constipation (FC)

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Alistipes
Bacteroidales
Bacteroidia
Bacteroidota
Betaproteobacteria
Comamonas
Fusobacterium
Streptococcus
TM73TM73
Candidatus Saccharibacteria

Revision editor(s): Tosin

Signature 2

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Curator: Tosin

Revision editor(s): Tosin

Source: Figure 3

Description: Differential abundant taxa between the constipation group and Healthy controls

Abundance in Group 1: decreased abundance in Patients with Functional constipation (FC)

NCBI	Quality Control	Links
Acinetobacter
Alphaproteobacteria
Anaerotruncus
Bacillales
Deferribacteraceae
Deferribacterales
Deferribacteres
Deferribacterota
Moraxellaceae
Mucispirillum
Oscillospira
Propionibacteriaceae
Propionibacterium
Pseudomonadales
Rickettsiales
unclassified Eubacteriales
unclassified Lachnospiraceae
unclassified Pseudomonadota
Muribaculaceae

Revision editor(s): Tosin