Association between premature ovarian insufficiency and gut microbiota

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

34090383

DOI Digital object identifier for electronic documents.

https://doi.org/10.1186/s12884-021-03855-w

URI Uniform resource identifier for web resources.

Authors

Wu J, Zhuo Y, Liu Y, Chen Y, Ning Y, Yao J

Journal

BMC pregnancy and childbirth

Year

2021

Keywords:

16S rRNA sequencing, Gut microbiota, Premature ovarian insufficiency, Sex hormones

BACKGROUND: Premature ovarian insufficiency (POI) is characterized by impairment of ovarian function on a continuum before the age of 40 years. POI is affected by multiple factors. Considering new insights from recent gut microbiome studies, this study aimed to investigate the relationship between gut microbial community structure and POI. METHODS: Subjects were recruited at the Shenzhen Maternity & Child Healthcare Hospital. Fecal microbial community profiles of healthy women (n = 18), women with POI (n = 35) were analyzed using 16S rRNA gene sequencing based on Illumina NovaSeq platform. RESULTS: Compared to the controls, the serum levels of FSH, LH, T and FSH/LH ratio significantly increased in women with POI, whereas E2 and AMH decreased significantly. Higher weighted UniFrac value was observed in POI women compared with healthy women. Phylum Firmicutes, genera Bulleidia and Faecalibacterium were more abundant in healthy women, while phylum Bacteroidetes, genera Butyricimonas, Dorea, Lachnobacterium and Sutterella enriched significantly in women with POI. Moreover, these alterations of the gut microbiome in women with POI were closely related to FSH, LH, E2, AMH level and FSH/LH ratio. CONCLUSIONS: Women with POI had altered microbial profiles in their gut microbiome, which were associated with serum hormones levels. These results will shed a new light on the pathogenesis and treatment for POI.

Experiment 1

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Curated date: 2025/07/29

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Primary ovarian insufficiency female hypergonadotropic hypogonadism,hypergonadotrophic ovarian failure,hypergonadotropic hypogonadism,hypergonadotropic hypogonadism (female),premature menopause,premature ovarian failure,premature ovarian insufficiency,primary female hypogonadism,primary ovarian failure,primary ovarian insufficiency,resistant ovary syndrome,Primary ovarian insufficiency

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Primary ovarian insufficiency (POI)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Thirty-five women with spontaneous Primary ovarian insufficiency (POI).

Group 0 sample size Number of subjects in the control (unexposed) group: 18

Group 1 sample size Number of subjects in the case (exposed) group: 35

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): Three months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Curated date: 2025/07/29

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 2C

Description: Significantly different and important microbes between the POI and the control group.

Abundance in Group 1: increased abundance in Primary ovarian insufficiency (POI)

NCBI	Quality Control	Links
Bacteroidota
Butyricimonas
Dorea
Lachnobacterium
Sutterella

Revision editor(s): Aleru Divine

Signature 2

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Curated date: 2025/07/29

Curator: Aleru Divine

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Source: Figure 2C

Description: Significantly different and important microbes between the POI and the control group.

Abundance in Group 1: decreased abundance in Primary ovarian insufficiency (POI)

NCBI	Quality Control	Links
Bacillota
Bulleidia
Faecalibacterium

Revision editor(s): Aleru Divine