The microbiomes of the eyelid and buccal area of patients with uveitic glaucoma

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Shin JH, Lee JW, Lim SH, Yoon BW, Lee Y, Seo JH
Journal
BMC ophthalmology
Year
2022
Keywords:
Buccal, Dysbiosis, Eyelid, Lactococcus, Microbiome, Uveitic glaucoma
BACKGROUND: The microbiome could trigger inflammation leading to epigenetic changes and is involved in the pathophysiology of eye diseases; however, its effect on uveitic glaucoma (UG) has not been fully investigated. This study analysed the differences in eyelid and buccal microbiomes in patients with UG using next-generation sequencing. METHODS: The eyelid and buccal specimens of 34 UG and 25 control patients were collected. The taxonomic composition of the microbiome was obtained via 16S ribosomal DNA sequencing. Diversity and differential gene expression analyses (DEG) determined taxon differences between the microbiomes of UG and control groups. RESULTS: In both the eyelid and buccal microbiomes, alpha-diversity was lower in UG patients than controls, while beta-diversity in patients with UG was higher than in controls. DEG analysis of the eyelid microbiome revealed various taxa differences, including enrichment of Paenibacillus and Dermacoccus (p-value, 1.31e-6 and 1.55e-7, respectively) and depletion of Morganella and Lactococcus (p-value, 6.26e-12 and 2.55e-6, respectively) in patients with UG. In the buccal microbiome, taxa such as Lactococcus was significantly depleted (p-value, 1.31e-17), whereas Faecalibacterium was enriched in patients with UG (p-value, 6.12e-8). CONCLUSIONS: The eyelid and buccal microbiomes in patients with UG differ from controls, which raises concerns surrounding environmental influences on the pathogenesis of UG. The reduced Lactococcus in the eyelid and buccal area suggest that microbiota dysbiosis is associated with UG.

Experiment 1


Needs review

Curated date: 2025/08/28

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Subjects

Location of subjects
South Korea
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Eyelid Blephara,Blepharon,Eye lid,Palpebra,Palpebrae,Eyelid,eyelid
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Glaucoma glaucoma,glaucoma (disease),Glaucoma (disorder),Glaucoma associated with other ocular disorders,Glaucoma associated with other ocular disorders (disorder),Glaucoma associated with other ocular disorders NOS,Glaucoma associated with other ocular disorders NOS (disorder),GLAUCOMA NEC,Glaucoma NOS,Glaucoma NOS (disorder),Glaucoma, NOS,Glaucomas,Other specified forms of glaucoma,Other specified forms of glaucoma (disorder),Other specified glaucoma,Other specified glaucoma NOS,Other specified glaucoma NOS (disorder),Unspecified glaucoma,Glaucoma
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
UG (Uveitic Glaucoma)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with Uveitic Glaucoma
Group 0 sample size Number of subjects in the control (unexposed) group
25
Group 1 sample size Number of subjects in the case (exposed) group
20
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.01
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2025/08/29

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 5, Supplementary Table S1

Description: Differentially abundant taxa between UG patients and control participants

Abundance in Group 1: increased abundance in UG (Uveitic Glaucoma)

NCBI Quality ControlLinks
Bacillariophyta
Bacteroides
Brevundimonas
Buttiauxella
Calidifontibacter
Candidatus Carsonella
Chryseobacterium
Citrobacter
Dermacoccus
Eggerthia
Escherichia/Shigella sp.
Faecalibacterium
Fontibacillus
Kandleria
Kocuria
Paenibacillus
Planctopirus
Roseburia
Soonwooa
Sulfophobococcus
Sulfurisphaera
Tepidimonas
Vampirovibrio
Vibrionimonas
Clostridium XVIIIClostridium XVIII

Revision editor(s): Anne-mariesharp

Signature 2

Needs review

Curated date: 2025/08/29

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 5, Supplementary Table S1

Description: Differentially abundant taxa between UG patients and control participants

Abundance in Group 1: decreased abundance in UG (Uveitic Glaucoma)

NCBI Quality ControlLinks
Bifidobacterium
Dermabacter
Dolosigranulum
Finegoldia
Halalkalibacillus
Klebsiella
Lactobacillus
Lactococcus
Moellerella
Morganella
Pluralibacter
Pseudocitrobacter
Psychrobacter
Serratia
Stenotrophomonas
Variovorax
Weissella
Xylella

Revision editor(s): Anne-mariesharp

Experiment 2


Needs review

Curated date: 2025/09/03

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Buccal mucosa Buccal mucosa,buccal mucosa
Group 1 sample size Number of subjects in the case (exposed) group
34

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2025/09/03

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 5, Supplementary Table S2

Description: Differentially abundant taxa between UG patients and control participants

Abundance in Group 1: increased abundance in UG (Uveitic Glaucoma)

NCBI Quality ControlLinks
Akkermansia
Alistipes
Anaerosphaera
Blautia
Candidatus Aenigmatarchaeota
Candidatus Carsonella
Collinsella
Coprobacillus
Dorea
Enterococcus
Faecalibacterium
Gemmiger
Kandleria
Kocuria
Lachnospiraceae incertae sedis
Megamonas
Parabacteroides
Peptostreptococcaceae incertae sedis
Phascolarctobacterium
Pseudomonas
Roseburia
Ruminococcus
Salarchaeum
Sulfophobococcus
Sulfurisphaera
Sutterella
Vampirovibrio
Woesearchaeota Incertae Sedis AR18Woesearchaeota Incertae Sedis AR18
Woesearchaeota Incertae Sedis AR20Woesearchaeota Incertae Sedis AR20
Pacearchaeota Incertae Sedis AR13Pacearchaeota Incertae Sedis AR13
Clostridium XVIIIClostridium XVIII
Clostridium XIClostridium XI
Woesearchaeota Incertae Sedis AR15Woesearchaeota Incertae Sedis AR15
Woesearchaeota Incertae Sedis AR16Woesearchaeota Incertae Sedis AR16
Ruminococcus2Ruminococcus2
Clostridium IVClostridium IV
Thermocladium
Methermicoccus
Alkalibaculum
Candidatus Parvarchaeum
Candidatus Scalindua
Acetobacteroides
Alcaligenes
Actinobaculum
Dichelobacter
Rhodocytophaga
Caldivirga
Clostridium XlVaClostridium XlVa
Thermogymnomonas

Revision editor(s): Anne-mariesharp

Signature 2

Needs review

Curated date: 2025/09/03

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Figure 5, Supplementary Table S2

Description: Differentially abundant taxa between UG patients and control participants

Abundance in Group 1: decreased abundance in UG (Uveitic Glaucoma)

NCBI Quality ControlLinks
Alkaliflexus
Anaeroarcus
Anaeromusa
Anaerosinus
Anaerosporobacter
Atopobacter
Atopobium
Atopococcus
Austwickia
Bavariicoccus
Beutenbergia
Bogoriella
Calidifontibacter
Candidatus Pelagibacter
Catellicoccus
Chlamydia
Dermabacter
Desemzia
Dolosigranulum
Eilatimonas
Garicola
Geobacillus
Geofilum
Georgenia
Granulicatella
Halalkalibacillus
Haloechinothrix
Humibacillus
Hydrogenimonas
Isobaculum
Jeotgalibaca
Lachnoanaerobaculum
Lactobacillus
Lactococcus
Leuconostoc
Luteipulveratus
Marihabitans
Megasphaera
Melissococcus
Miniimonas
Morganella
Moryella
Negativicoccus
Oribacterium
Ornithinicoccus
Owenweeksia
Parascardovia
Pilibacter
Piscicoccus
Pisciglobus
Prevotella
Psychrobacter
Psychrosinus
Salana
Sciscionella
Sedimenticola
Sediminihabitans
Solobacterium
Stomatobaculum
Tetragenococcus
Veillonella
Marinimicrobia_genera_incertae_sedisMarinimicrobia_genera_incertae_sedis
Lacticigenium
Stenotrophomonas
Kineosphaera
Anoxybacillus
Psychrilyobacter
Finegoldia
Lactovum

Revision editor(s): Anne-mariesharp