Relationship between gut microbiome characteristics and the effect of nutritional therapy on glycemic control in pregnant women with gestational diabetes mellitus
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Chen J, Yang Y, Yu N, Sun W, Yang Y, Zhao M
Journal
PloS one
Year
2022
The purpose of this study was to explore the relationship between the characteristics of gut microbiome and the effect of medical nutrition therapy (MNT) on glycemic control in pregnant women with gestational diabetes mellitus (GDM). Seventy-four pregnant women newly diagnosed with GDM received MNT for one-week. The effect of glycemic control was evaluated by fasting and 2-hour postprandial blood glucose; and stool samples of pregnant women were collected to detect the gut microbiome before and after MNT. We used a nested case-control study design, with pregnant women with GDM who did not meet glycemic standards after MNT as the ineffective group and those with an age difference of ≤5 years, matched for pre-pregnancy body mass index (BMI) 1:1, and meeting glycemic control criteria as the effective group. Comparison of the gut microbiome characteristics before MNT showed that the ineffective group was enriched in Desulfovibrio, Aeromonadales, Leuconostocaceae, Weissella, Prevotella, Bacillales_Incertae Sedis XI, Gemella and Bacillales, while the effective group was enriched in Roseburia, Clostridium, Bifidobacterium, Bifidobacteriales, Bifidobacteriaceae, Holdemania and Proteus. After treatment, the effective group was enriched in Bifidobacterium and Actinomycete, while the ineffective group was enriched in Holdemania, Proteus, Carnobacteriaceae and Granulicatella. In conclusion, the decrease in the abundance of characteristic gut microbiome positively correlated with blood glucose may be a factor influencing the poor hypoglycemic effect of MNT in pregnant women with GDM. Abundance of more characteristic gut microbiome negatively correlated with blood glucose could help control blood glucose in pregnant women with GDM.
Experiment 1
Empty strings are not accepted.
Needs review
Curated date: 2025/02/04
Curator:
Revision editor(s):
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Maternal diabetes
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Ineffective group before the therapy (N1)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Effective group before the therapy (Y1)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The Y1 group consisted of pregnant women with Gestational Diabetes Mellitus (GDM) whose glycemic control effect was on target before medical nutrition therapy (MNT).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 62
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 20 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.0
- Matched on Factors on which subjects have been matched on in a case-control study
- age, body mass index
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Needs review
Source: Figure 2B
Description: Bacterial signatures that are statistically different between the effective and ineffective groups before medical nutrition therapy (MNT).
Abundance in Group 1: increased abundance in
| NCBI | Quality Control | Links |
|---|---|---|
| Roseburia | ||
| Clostridium XIVaClostridium XIVa | ||
| Bifidobacteriales | ||
| Bifidobacteriaceae | ||
| Bifidobacterium | ||
| Holdemania | ||
| Proteus |
Revision editor(s): Aleru Divine, WikiWorks
Signature 2
Needs review
Source: Figure 2B
Description: Bacterial signatures that are statistically different between the effective and ineffective groups before medical nutrition therapy (MNT).
Abundance in Group 1: decreased abundance in
| NCBI | Quality Control | Links |
|---|---|---|
| Aeromonadales | ||
| Alloprevotella | ||
| Bacillales | ||
| Desulfovibrio | ||
| Gemella | ||
| Lactobacillaceae | ||
| Weissella | ||
| Gemellaceae |
Revision editor(s): Aleru Divine, WikiWorks
Experiment 2
Needs review
Curated date: 2025/02/04
Curator:
Revision editor(s):
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Ineffective group after the therapy (N2)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Effective group after the therapy (Y2)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The Y2 group consisted of pregnant women with Gestational Diabetes Mellitus (GDM) whose glycemic control effect was on target after medical nutrition therapy (MNT).
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Needs review
Source: Figure 5C
Description: Bacterial signatures that are statistically different between the effective and ineffective groups after medical nutrition therapy (MNT).
Abundance in Group 1: increased abundance in
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Bifidobacteriaceae | ||
| Bifidobacteriales | ||
| Actinomycetota | ||
| Acidimicrobiia |
Revision editor(s): Aleru Divine, WikiWorks
Signature 2
Needs review
Source: Figure 5C
Description: Bacterial signatures that are statistically different between the effective and ineffective groups after medical nutrition therapy (MNT).
Abundance in Group 1: decreased abundance in
| NCBI | Quality Control | Links |
|---|---|---|
| Holdemania | ||
| Proteus | ||
| Carnobacteriaceae | ||
| Granulicatella |
Revision editor(s): Aleru Divine, WikiWorks
Experiment 3
Needs review
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Effective group before the therapy (Y1)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 62
Lab analysis
Statistical Analysis
- Matched on Factors on which subjects have been matched on in a case-control study
- Not specified
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Needs review
Source: Figure 4C
Description: Results of linear discriminant analysis of gut microbiome before and after therapy outcome between effective group.
Abundance in Group 1: decreased abundance in Effective group after the therapy (Y2)
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillibacter |
Revision editor(s): Aleru Divine, WikiWorks
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