High-Fat Diet Enhances the Liver Metastasis Potential of Colorectal Cancer through Microbiota Dysbiosis

Study information

Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

study design

laboratory experiment
case-control

Citation

PMID PubMed identifier for scientific articles.

35681554

DOI Digital object identifier for electronic documents.

10.3390/cancers14112573

URI

Authors

Yu Y, Cai Y, Yang B, Xie S, Shen W, Wu Y, Sui Z, Cai J, Ni C, Ye J

Journal

Cancers

Year

2022

Keywords:

Desulfovibrio, colorectal cancer, high-fat diet, liver metastasis, microbiota dysbiosis, tumour microenvironment

Obesity, metabolic changes, and intestinal microbiota disruption significantly affect tumorigenesis and metastasis in colorectal cancer (CRC). However, the relationships among these factors remain poorly understood. In this study, we found that a high-fat diet (HFD) promoted gut barrier dysfunction and inflammation in the colorectum and liver. We further investigated gut microbiota changes through 16S rRNA sequencing of faecal samples from HFD-fed rats and CRC hepatic metastasis patients and found an abundance of Desulfovibrio (DSV). DSV could also induce barrier dysfunction in the colorectum and inflammation in the colorectum and liver, suggesting that it contributes to the formation of a microenvironment conducive to CRC tumorigenesis and metastasis. These findings highlight that HFD-induced microbiota dysbiosis, especially DSV abundance, could promote CRC initiation and metastasis.

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/03/23

Curator: ShadeAkinremi

Revision editor(s): ShadeAkinremi, KateRasheed

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Rattus norvegicus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: High fat diet HF - High fat diet,High fat diet (finding),High fat diet,high fat diet

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: High fat diet

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: This refers to adult male Wistar rats and adult male BALB/c mice; who were fed with high fat diet.

Group 0 sample size Number of subjects in the control (unexposed) group: 7

Group 1 sample size Number of subjects in the case (exposed) group: 7

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Signature 1

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Curated date: 2025/03/23

Curator: ShadeAkinremi

Revision editor(s): ShadeAkinremi, KateRasheed

Source: Figure 4C, 4D, 4E, 5G

Description: Comparison of fecal bacterial composition at the phylum and genus levels between rats subjected to eight weeks of high-fat diet treatment and healthy controls.

Abundance in Group 1: increased abundance in High fat diet

NCBI	Quality Control	Links
Acetatifactor
Bacteroidota
Christensenella
Desulfovibrio
Enterorhabdus
Helicobacter
Olsenella
Oscillibacter
Pseudomonadota
Ruminococcus
unclassified Bacillota
Clostridium_XlVbClostridium_XlVb
Pseudomonas
unclassified Desulfovibrionales

Revision editor(s): ShadeAkinremi, KateRasheed

Signature 2

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Curated date: 2025/03/24

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Source: Figure 4E

Description: Comparison of fecal bacterial composition at the phylum and genus levels between rats subjected to eight weeks of high-fat diet treatment and healthy controls.

Abundance in Group 1: decreased abundance in High fat diet

NCBI	Quality Control	Links
Prevotella
unclassified Veillonellaceae
Anaerotruncus

Revision editor(s): ShadeAkinremi

Experiment 2

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Differences from previous experiment shown

Subjects

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Metastatic colorectal cancer Metastatic colorectal cancer,metastatic colorectal cancer

Group 0 name Corresponds to the control (unexposed) group for case-control studies: non-metastatic group of colorectal cancer

Group 1 name Corresponds to the case (exposed) group for case-control studies: metastatic group of colorectal cancer

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Colorectal cancer patients with liver metastases.

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 13

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 1 month

Statistical Analysis

Signature 1

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Source: Figure 5C

Description: Comparison of fecal bacterial composition at the phylum and genus levels between colorectal cancer patients with and without liver metastases.

Abundance in Group 1: increased abundance in metastatic group of colorectal cancer

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Barnesiella
Desulfovibrio
Gemmiger
Ruminococcus

Revision editor(s): ShadeAkinremi