Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment
From BugSigDB
Jump to:navigation, search
Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Tsakeng CUB, Tanekou TTM, Soffack SF, Tirados I, Noutchih C, Njiokou F, Bigoga JD, Wondji CS
Journal
Microorganisms
Year
2022
Keywords:
microbiome, trypanosomes, tsetse flies, vector competence, vector control
The tsetse flies, biological vectors of African trypanosomes, harbour a variety of bacteria involved in their vector competence that may help in developing novel vector control tools. This study provides an inventory of tsetse bacterial communities in Cameroon and explores their possible associations with trypanosome establishment in Glossina palpalis palpalis. High throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene, with subsequent metagenomic, multivariate, and association analyses, were used to investigate the levels and patterns of microbial diversity in four tsetse species. Overall, 31 bacterial genera and four phyla were identified. The primary symbiont Wigglesworthia dominated almost all the samples, with an overall relative abundance of 47.29%, and seemed to be replaced by Serratia or Burkholderia in some G. tachinoides flies. Globally, significant differences were observed in the microbiome diversity and composition among tsetse species and between teneral and non-teneral flies, or between flies displaying or not displaying mature trypanosome infections. In addition, differential abundance testing showed some OTUs, or some bacteria taxa, associated with trypanosome maturation in tsetse flies. These bacteria could be further investigated for an understanding of their mechanism of action and alternatively, transformed and used to block trypanosome development in tsetse flies.
Experiment 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Subjects
- Location of subjects
- Cameroon
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Glossina palpalis palpalis
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Insect head , Body proper Insect head,insect head,Body,Whole body,Body proper,body proper
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Human african trypanosomiasis Africam sleeping sickness,African sleeping sickness,African Trypanosomiasis,African trypanosomiasis,human African trypanosomiasis,sleeping sickness,Human african trypanosomiasis,human african trypanosomiasis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-infected
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Infected
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Tsetse flies with midguts infected with trypanosomes.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Source: Table 2
Description: Operational Taxonomic Units (at the genus level) displaying a significant difference in abundance between tsetse flies with midguts non-infected vs. infected with trypanosomes.
Abundance in Group 1: increased abundance in Infected
| NCBI | Quality Control | Links |
|---|---|---|
| Serratia | ||
| Staphylococcus | ||
| Wigglesworthia |
Revision editor(s): Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Source: Table 2
Description: Operational Taxonomic Units (at the genus level) displaying a significant difference in abundance between tsetse flies with midguts non-infected vs. infected with trypanosomes.
Abundance in Group 1: decreased abundance in Infected
| NCBI | Quality Control | Links |
|---|---|---|
| Aquabacterium | ||
| Bacillus | ||
| Cupriavidus | ||
| Dechloromonas | ||
| Enterococcus | ||
| Listeria | ||
| Methylophilus | ||
| Pseudomonas | ||
| Ralstonia | ||
| Pseudomonas_ EscherichiaPseudomonas_ Escherichia | ||
| Roseateles |
Revision editor(s): Anne-mariesharp
Experiment 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Disease staging disease_staging,Disease staging,disease staging
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-mature infection
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mature infection
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Tsetse flies with mature infection with trypanosomes.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Source: Table 3
Description: Operational Taxonomic Units (in the genus level) displaying a significant difference in abundance between tsetse flies with non-mature vs. mature infection with trypanosomes.
Abundance in Group 1: increased abundance in Mature infection
| NCBI | Quality Control | Links |
|---|---|---|
| Bacillus | ||
| Enhydrobacter |
Revision editor(s): Montana-D
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Source: Table 3
Description: Operational Taxonomic Units (at the genus level) displaying a significant difference in abundance between tsetse flies with non-mature vs. mature infection with trypanosomes.
Abundance in Group 1: decreased abundance in Mature infection
| NCBI | Quality Control | Links |
|---|---|---|
| Ralstonia | ||
| Serratia | ||
| Staphylococcus |
Revision editor(s): Montana-D
Retrieved from "https://bugsigdb.org/w/index.php?title=35744659&oldid=206111"
