Alleviating effects of gut micro-ecologically regulatory treatments on mice with constipation

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/15

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, KateRasheed

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Control group

Group 1 name Corresponds to the case (exposed) group for case-control studies: Model group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice in Model group was orally treated only with saline per day during the experimental period.

Group 0 sample size Number of subjects in the control (unexposed) group: 8

Group 1 sample size Number of subjects in the case (exposed) group: 8

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/15

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: increased abundance in Model group

NCBI	Quality Control	Links
Akkermansia

Revision editor(s): PreciousChijioke

Experiment 4

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Model group

Group 1 name Corresponds to the case (exposed) group for case-control studies: Mice in MP (mixed probiotics) group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice in MP (mixed probiotics) group was treated every day with loperamide (10 mg/kg body weight), and with corresponding products (Mixed probiotics) 1 h after loperamide administration.

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: increased abundance in Mice in MP (mixed probiotics) group

NCBI	Quality Control	Links
Eubacterium xylanophilum
Ruminiclostridium 6Ruminiclostridium 6
Ruminiclostridium 9Ruminiclostridium 9

Revision editor(s): PreciousChijioke

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: decreased abundance in Mice in MP (mixed probiotics) group

NCBI	Quality Control	Links
Alistipes

Revision editor(s): PreciousChijioke

Experiment 5

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: Mice in MPP (mixed probiotics plus postbiotics) group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice in MPP (mixed probiotics plus postbiotics) group was treated every day with loperamide (10 mg/kg body weight), and with corresponding products (Mixed probiotics plus postbiotics) 1 h after loperamide administration.

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: increased abundance in Mice in MPP (mixed probiotics plus postbiotics) group

NCBI	Quality Control	Links
Eubacterium xylanophilum
Ruminiclostridium 6Ruminiclostridium 6
Ruminiclostridium 9Ruminiclostridium 9

Revision editor(s): PreciousChijioke

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: decreased abundance in Mice in MPP (mixed probiotics plus postbiotics) group

NCBI	Quality Control	Links
Alistipes

Revision editor(s): PreciousChijioke

Experiment 6

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: Mice in P (postbiotics) group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice in P (postbiotics) group was treated every day with loperamide (10 mg/kg body weight), and with corresponding products (Postbiotics) 1 h after loperamide administration.

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: increased abundance in Mice in P (postbiotics) group

NCBI	Quality Control	Links
Bacteroides
Ruminiclostridium
Ruminiclostridium 6Ruminiclostridium 6
Ruminiclostridium 9Ruminiclostridium 9
Ruminococcus 1Ruminococcus 1

Revision editor(s): PreciousChijioke

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: decreased abundance in Mice in P (postbiotics) group

NCBI	Quality Control	Links
Alistipes

Revision editor(s): PreciousChijioke

Experiment 7

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: Mice in P5 (5-fold dose of postbiotics) group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice in P5 (5-fold dose of postbiotics) group was treated every day with loperamide (10 mg/kg body weight), and with corresponding products (Postbiotics) 1 h after loperamide administration.

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: increased abundance in Mice in P5 (5-fold dose of postbiotics) group

NCBI	Quality Control	Links
Bacteroides
Ruminiclostridium
Ruminiclostridium 6Ruminiclostridium 6
Ruminiclostridium 9Ruminiclostridium 9
Ruminococcus 1Ruminococcus 1

Revision editor(s): PreciousChijioke

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: decreased abundance in Mice in P5 (5-fold dose of postbiotics) group

NCBI	Quality Control	Links
Alistipes

Revision editor(s): PreciousChijioke

Experiment 8

Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies: S (synbiotics) group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: S (synbiotics) group was treated every day with loperamide (10 mg/kg body weight), and with corresponding products (Synbiotics) 1 h after loperamide administration.

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: increased abundance in S (synbiotics) group

NCBI	Quality Control	Links
Bacteroides
Ruminiclostridium
Ruminiclostridium 6Ruminiclostridium 6
Ruminiclostridium 9Ruminiclostridium 9
Ruminococcus 1Ruminococcus 1

Revision editor(s): PreciousChijioke

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-16

Curated date: 2025/04/11

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke

Source: "Within-text result of "The synbiotics changed gut microbial composition in mice" section"

Description: Heatmap of the top 20 fecal microbial genera among groups.

Abundance in Group 1: decreased abundance in S (synbiotics) group

NCBI	Quality Control	Links
Alistipes

Revision editor(s): PreciousChijioke

Experiment 9