Gut microbiota composition in chemotherapy and targeted therapy of patients with metastatic colorectal cancer
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- Taiwan
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Colorectal cancer cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine,Colorectal cancer
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Partial response group- PR
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Progressive disease group- PD
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients who had a progressive disease (PD) for at least 12 cycles of therapy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 31
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
Signature 1
Source: Figure 4A
Description: Results of the log2 fold change in the disease progression and partial response groups
Abundance in Group 1: increased abundance in Progressive disease group- PD
Revision editor(s): Miss Lulu
Signature 2
Source: Figure 4A
Description: Results of the log2 fold change in the disease progression and partial response groups
Abundance in Group 1: decreased abundance in Progressive disease group- PD
Revision editor(s): Miss Lulu
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Bevacizumab-PR subgroup
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bevacizumab-PD subgroup
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with progressive disease (PD) who had anti-vascular endothelial growth factor (bevacizumab) subgroups.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 17
- Group 1 sample size Number of subjects in the case (exposed) group
- 18
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
Signature 1
Source: Figure 4B
Description: Results of the log2 fold change in the subgroups of bevacizumab
Abundance in Group 1: increased abundance in Bevacizumab-PD subgroup
Revision editor(s): Miss Lulu
Signature 2
Source: Figure 4B
Description: Results of the log2 fold change in the subgroups of bevacizumab
Abundance in Group 1: decreased abundance in Bevacizumab-PD subgroup
NCBI | Quality Control | Links |
---|---|---|
Segatella copri | ||
Prevotella dentalis | ||
Phocaeicola coprophilus |
Revision editor(s): Miss Lulu
Experiment 3
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Cetuximab-PR subgroup
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Cetuximab-PD subgroup
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with progressive disease (PD) who had anti-epidermal growth factor receptor (cetuximab) subgroups.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 14
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Figure 4C
Description: Results of the log2 fold change in the subgroups of cetuximab
Abundance in Group 1: increased abundance in Cetuximab-PD subgroup
NCBI | Quality Control | Links |
---|---|---|
Anaerostipes caccae | ||
Adlercreutzia equolifaciens |
Revision editor(s): Miss Lulu