Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by Ligilactobacillus salivarius Li01/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-6-10
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Intestine secretion Intestinal juice,Intestinal secretion,Succus entericus,Intestine secretion,intestine secretion
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NC (Normal Control)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- LOP (Loperamide-induced constipation)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice that received a daily gavage of 0.4 mL saline buffer for 15 days and 0.2 mL loperamide hydrochloride (10 mg/kg body weight) from the 8th to 15th day to induce constipation.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- ANOVA
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-10
Source: Figure 5D
Description: Differentially abundant taxon between the NC (Normal Control) group and LOP (Loperamide-induced constipation) group.
Abundance in Group 1: increased abundance in LOP (Loperamide-induced constipation)
| NCBI | Quality Control | Links |
|---|---|---|
| Muribaculaceae |
Revision editor(s): Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-10
Source: Figure 5E
Description: Differentially abundant taxa between the NC (Normal Control) group and LOP (Loperamide-induced constipation) group.
Abundance in Group 1: decreased abundance in LOP (Loperamide-induced constipation)
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Lachnospiraceae bacterium NK4A136 | ||
| Ruminococcus |
Revision editor(s): Anne-mariesharp
