Multi-omics analyses reveal the specific changes in gut metagenome and serum metabolome of patients with polycystic ovary syndrome/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-7-3
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Polycystic ovary syndrome Cystic disease of ovaries,hyperandrogenemia,Multicystic ovaries,multicystic ovaries,Ovarian Degeneration, Sclerocystic,Ovarian Syndrome, Polycystic,Ovarian Syndromes, Polycystic,Ovaries, Sclerocystic,Ovary Syndrome, Polycystic,Ovary, Sclerocystic,PCO - Polycystic ovaries,Pco1,PCOD - Polycystic ovarian disease,PCOS,Pcos,PCOS - Polycystic ovarian syndrome,PCOS1,Polycystic ovarian disease,polycystic ovarian disease,Polycystic ovarian syndrome,Polycystic ovaries,polycystic ovaries,Polycystic ovaries (disorder),polycystic ovary,polycystic ovary syndrome,polycystic ovary syndrome 1,Sclerocystic Ovarian Degeneration,Sclerocystic Ovaries,Sclerocystic Ovary,Sclerocystic Ovary Syndrome,Stein Leventhal Syndrome,Stein-Leventhal synd.,Stein-Leventhal Syndrome,Stein-Leventhal syndrome,Syndrome, Polycystic Ovary,Syndrome, Stein-Leventhal,Polycystic ovary syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy individuals (Control)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Polycystic ovary syndrome (PCOS)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients diagnosed with polycystic ovary syndrome (PCOS) according to the Rotterdam criteria, which include at least two of the following: oligo- or anovulation, clinical/biochemical signs of hyperandrogenism, and polycystic ovaries detected by ultrasound.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 18
- Group 1 sample size Number of subjects in the case (exposed) group
- 32
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 Month
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-3
Source: Figure S2B and S3B
Description: Different microbial genera with abundance of over 0.05% between PCOS and healthy controls. The comparison between two groups is performed by the two-tailed Mann-Whitney U-test, and P value is adjusted by Benjamini-Hochberg method.
Abundance in Group 1: increased abundance in Polycystic ovary syndrome (PCOS)
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia | ||
| Actinomycetota | ||
| Actinomyces | ||
| Enterococcus | ||
| Coprobacillus | ||
| Pseudomonas | ||
| Thomasclavelia | ||
| Gordonibacter |
Revision editor(s): Shulamite
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-3
Source: Figure S2B and S3B
Description: Different microbial genera with abundance of over 0.05% between PCOS and healthy controls. The comparison between two groups is performed by the two-tailed Mann-Whitney U-test, and P value is adjusted by Benjamini-Hochberg method.
Abundance in Group 1: decreased abundance in Polycystic ovary syndrome (PCOS)
| NCBI | Quality Control | Links |
|---|---|---|
| Faecalibacterium | ||
| Odoribacter | ||
| Parabacteroides | ||
| Paraprevotella | ||
| Phascolarctobacterium | ||
| Roseburia |
Revision editor(s): Shulamite
