Cannabis sativa L. alleviates loperamide-induced constipation by modulating the composition of gut microbiota in mice
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Traditional Chinese medicine type TCM type,Traditional Chinese medicine type,traditional Chinese medicine type
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Combination of Con (blank) and Lop + WECSL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L.)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Lop (Loperamide hydrochloride-induced constipation model)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice injected with loperamide (10.0 mg/kg) twice a day for 2 weeks
- Group 0 sample size Number of subjects in the control (unexposed) group
- 16
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Signature 1
Source: Figure 6A and 6B
Description: Identification of most characteristic taxa among experimental groups by linear discriminant analysis (LDA) effect size (LEfSe).
Abundance in Group 1: increased abundance in Lop (Loperamide hydrochloride-induced constipation model)
NCBI | Quality Control | Links |
---|---|---|
Parabacteroides | ||
Parasutterella | ||
Blautia | ||
TuzzerellaTuzzerella | ||
Gordonibacter | ||
Clostridium | ||
[Eubacterium] brachy |
Revision editor(s): Tosin
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Combination of Con (blank) and Lop (Loperamide hydrochloride-induced constipation model)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Lop + WESCL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L. )
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Constipation mice pretreated with the water extract of Cannabis sativa L. (WECSL)
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 6A and 6B
Description: Identification of most characteristic taxa among experimental groups by linear discriminant analysis (LDA) effect size (LEfSe).
Abundance in Group 1: increased abundance in Lop + WESCL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L. )
NCBI | Quality Control | Links |
---|---|---|
Allobaculum | ||
Anaerotruncus | ||
Butyricicoccus | ||
Monoglobus | ||
Muribaculum | ||
Paraprevotella | ||
Prevotella | ||
Tyzzerella | ||
Termite treponema clusterTermite treponema cluster |
Revision editor(s): Tosin
Experiment 3
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Combination of Lop (Loperamide hydrochloride-induced constipation model) and Lop +WESCL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L.)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Con (blank)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice in the Con group were injected with the same volume of saline
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 6A and 6B
Description: Identification of most characteristic taxa among experimental groups by linear discriminant analysis (LDA) effect size (LEfSe).
Abundance in Group 1: increased abundance in Con (blank)
Revision editor(s): Tosin