Cannabis sativa L. alleviates loperamide-induced constipation by modulating the composition of gut microbiota in mice

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Li R, Li M, Li B, Chen WH, Liu Z
Journal
Frontiers in pharmacology
Year
2022
Keywords:
Butyricicoccus, Cannabis sativa L., Parasutterella, constipation, gut microbiota
MaZiRenWan (MZRW) is the most frequently used Traditional Chinese Medicine formula to treat chronic constipation, Cannabis sativa L. is regarded as a monarch drug in MZRW. However, the targets of Cannabis sativa L. that enhance colonic motility and improve constipation symptoms remain unknown. This study was designed to investigate the laxative effect and underlying mechanism of the water extract of Cannabis sativa L. (WECSL) using a loperamide-induced constipation mouse model. We found that WECSL treatment significantly improved intestinal motility and water-electrolyte metabolism, decreased inflammatory responses, prevented gut barrier damage, and relieved anxiety and depression in constipated mice. WECSL also structurally remodeled the composition of the gut microbiota and altered the abundance of bacteria related to inflammation, specifically Butyricicoccus and Parasutterella. Moreover, WECSL failed to relieve constipation symptoms following intestinal flora depletion, indicating that WECSL alleviates constipation symptoms depending on the gut microbiota. Our research provides a basis for WECSL to be further investigated in the treatment of constipation from the perspective of modern medicine.

Experiment 1


Needs review

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Traditional Chinese medicine type TCM type,Traditional Chinese medicine type,traditional Chinese medicine type
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of Con (blank) and Lop + WECSL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L.)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Lop (Loperamide hydrochloride-induced constipation model)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice injected with loperamide (10.0 mg/kg) twice a day for 2 weeks
Group 0 sample size Number of subjects in the control (unexposed) group
16
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3


Signature 1

Needs review

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 6A and 6B

Description: Identification of most characteristic taxa among experimental groups by linear discriminant analysis (LDA) effect size (LEfSe).

Abundance in Group 1: increased abundance in Lop (Loperamide hydrochloride-induced constipation model)

NCBI Quality ControlLinks
Parabacteroides
Parasutterella
Blautia
TuzzerellaTuzzerella
Gordonibacter
Clostridium
[Eubacterium] brachy

Revision editor(s): Tosin

Experiment 2


Needs review

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of Con (blank) and Lop (Loperamide hydrochloride-induced constipation model)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Lop + WESCL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L. )
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Constipation mice pretreated with the water extract of Cannabis sativa L. (WECSL)

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 6A and 6B

Description: Identification of most characteristic taxa among experimental groups by linear discriminant analysis (LDA) effect size (LEfSe).

Abundance in Group 1: increased abundance in Lop + WESCL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L. )

NCBI Quality ControlLinks
Allobaculum
Anaerotruncus
Butyricicoccus
Monoglobus
Muribaculum
Paraprevotella
Prevotella
Tyzzerella
Termite treponema clusterTermite treponema cluster

Revision editor(s): Tosin

Experiment 3


Needs review

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of Lop (Loperamide hydrochloride-induced constipation model) and Lop +WESCL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L.)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Con (blank)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice in the Con group were injected with the same volume of saline

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 6A and 6B

Description: Identification of most characteristic taxa among experimental groups by linear discriminant analysis (LDA) effect size (LEfSe).

Abundance in Group 1: increased abundance in Con (blank)

NCBI Quality ControlLinks
Alistipes
Anaeroplasma
Bacteroides
Bilophila
Candidatus Saccharimonas
Intestinimonas
Lachnoclostridium
Muribaculaceae
Clostridia vadinBB60 groupClostridia vadinBB60 group
Lachnospiraceae UCG 004Lachnospiraceae UCG 004
Lachnospiraceae ASF356Lachnospiraceae ASF356
Oscillospirales UCG 010Oscillospirales UCG 010

Revision editor(s): Tosin