Alteration of faecal microbiota balance related to long-term deep meditation/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-1-3
Curated date: 2023/10/05
Curator: Nwajei Edgar
Revision editor(s): WikiWorks, LGeistlinger, Deacme, Nwajei Edgar
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Exercise Exercise,exercise
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Meditation group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Tibetan Buddhist monks that performed meditation practices of Samatha and Vipassana for at least 2 hours a day for 3 to 30 years.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 19
- Group 1 sample size Number of subjects in the case (exposed) group
- 37
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3.6
- Matched on Factors on which subjects have been matched on in a case-control study
- age, alcohol drinking, diet, sex, smoking status
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- decreased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-3
Source: Figure 4
Description: Relative abundances of gut microbiota and linear discriminant analysis (LDA) effect size (LEfSe) in the meditation and control groups.
Abundance in Group 1: increased abundance in Meditation group
| NCBI | Quality Control | Links |
|---|---|---|
| Faecalibacterium | ||
| Megamonas | ||
| Prevotella | ||
| Bacteroides | ||
| Bacteroidales | ||
| Sutterellaceae | ||
| Burkholderiales | ||
| Betaproteobacteria |
Revision editor(s): Nwajei Edgar, Deacme, WikiWorks
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-3
Curated date: 2023/10/05
Curator: Nwajei Edgar
Revision editor(s): WikiWorks, Deacme, Nwajei Edgar, Folakunmi
Source: Figure 4
Description: Relative abundances of gut microbiota and linear discriminant analysis (LDA) effect size (LEfSe) in the meditation and control groups.
Abundance in Group 1: decreased abundance in Meditation group
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Candidatus Saccharimonadota | ||
| Collinsella | ||
| Dialister | ||
| Megasphaera | ||
| Roseburia | ||
| Streptophyta | ||
| Veillonellaceae | ||
| Actinomycetota | ||
| Gammaproteobacteria |
Revision editor(s): WikiWorks, Deacme, Nwajei Edgar, Folakunmi
