Breast Cancer Survivors and Healthy Women: Could Gut Microbiota Make a Difference?-"BiotaCancerSurvivors": A Case-Control Study

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Caleça T, Ribeiro P, Vitorino M, Menezes M, Sampaio-Alves M, Mendes AD, Vicente R, Negreiros I, Faria A, Costa DA
Journal
Cancers
Year
2023
Keywords:
breast cancer, cancer, case-control study, microbiome, microbiota, survivors, survivorship
In this first analysis, samples from 23 BC survivors (group 1) and 291 healthy female controls (group 2) were characterised through the V3 and V4 regions that encode the "16S rRNA" gene of each bacteria. The samples were sequenced by next-generation sequencing (NGS), and the taxonomy was identified by resorting to Kraken2 and improved with Bracken, using a curated database called 'GutHealth_DB'. The α and β-diversity analyses were used to determine the richness and evenness of the gut microbiota. A non-parametric Mann-Whitney U test was applied to assess differential abundance between both groups. The Firmicutes/Bacteroidetes (F/B) ratio was calculated using a Kruskal-Wallis chi-squared test. The α-diversity was significantly higher in group 1 (p = 0.28 × 10-12 for the Chao index and p = 1.64 × 10-12 for the ACE index). The Shannon index, a marker of richness and evenness, was not statistically different between the two groups (p = 0.72). The microbiota composition was different between the two groups: a null hypothesis was rejected for PERMANOVA (p = 9.99 × 10-5) and Anosim (p = 0.04) and was not rejected for β-dispersion (p = 0.158), using Unifrac weighted distance. The relative abundance of 14 phyla, 29 classes, 25 orders, 64 families, 116 genera, and 74 species differed significantly between both groups. The F/B ratio was significantly lower in group 1 than in group 2, p < 0.001. Our study allowed us to observe significant taxonomic disparities in the two groups by testing the differences between BC survivors and healthy controls. Additional studies are needed to clarify the involved mechanisms and explore the relationship between microbiota and BC survivorship.

Experiment 1


Needs review

Curated date: 2025/07/11

Curator: Ecsharp

Revision editor(s): Ecsharp

Subjects

Location of subjects
Portugal
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Breast cancer breast cancer,breast tumor,cancer of breast,malignant breast neoplasm,malignant breast tumor,malignant neoplasm of breast,malignant neoplasm of the breast,malignant tumor of breast,malignant tumor of the breast,mammary cancer,mammary neoplasm,mammary tumor,primary breast cancer,Breast cancer
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy female controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
BC survivors
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Women aged over 18 with a breast cancer (BC) diagnosis who had completed their core treatments (surgery, chemotherapy, and/or radiotherapy).
Group 0 sample size Number of subjects in the control (unexposed) group
291
Group 1 sample size Number of subjects in the case (exposed) group
23

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Ion Torrent, Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased

Signature 1

Needs review

Curated date: 2025/07/11

Curator: Ecsharp

Revision editor(s): Ecsharp

Source: Table 2 & Section 3.3: Microbiota Analysis

Description: Table 2 consists of relative abundance of specific bacterial groups in stools of BC survivors and control group by specific primers and Section 3.3. Microbiota Analysis discusses further significant relative abundance results in text. (p value of ≤ 0.05, Mann-Whitney U test)

Abundance in Group 1: increased abundance in BC survivors

NCBI Quality ControlLinks
Acetobacterium
Acidaminobacter
Acidaminococcus
Alloprevotella tannerae
Anaerofilum
Arcobacter
Armatimonadota
Bacteroides nordii
Brachymonas
Campylobacter rectus
Candidatus Neoarthromitus
Candidatus Latescibacterota
Candidatus Saccharimonadota
Chloroflexota
Citrobacter freundii
Coprobacillus cateniformis
Desulfurispora
Enterobacter kobei
Enterocloster clostridioformis
Fibrobacterota
Franconibacter
Frigoribacterium
Gemmatimonadota
Gluconobacter oxydans
Hungatella hathewayi
Idiomarina
Kitasatospora
Lacrimispora aerotolerans
Legionella
Macrococcus
Marinomonas
Microcoleus
Mitsuokella multacida
Natronincola
Nitrospirota
Paludibacter
Pandoraea
Paraclostridium sordellii
Photorhabdus
Prevotella intermedia
Prevotella nigrescens
Prevotella pallens
Renibacterium
Roseateles
Roseateles depolymerans
Scardovia
Sharpea
Shigella
Shigella flexneri
Shigella sonnei
Sphaerochaeta
Spirochaetota
Streptococcus lutetiensis
Sulfurimonas
Thermoanaerobacterium
Tissierella
Verrucomicrobiota
OP11OP11
OP8OP8
Fidelibacterota
AF12AF12
CF231CF231
GW-34GW-34
Neomoorella
Oceanicaulis
TG5TG5
Soehngenia
vadinHB04vadinHB04
ZA3312cZA3312c
Clostridium acetobutylicum
Desulforamulus aeronauticus
Lacrimispora algidixylanolytica
Litchfieldella anticariensis
Vibrio atlanticus
Paraclostridium bifermentans
Liquorilactobacillus capillatus
Franconibacter daqui
Brachymonas denitrificans
Clostridium intestinale
Virgibacillus marismortui
Edwardsiella piscicida
Kitasatospora pitsanulaokmensis
Pseudomonas psychrophila
Kosakonia radicincitans
Thermoanaerobacterium saccharolyticum
Clostridium sartagoforme
Pseudarthrobacter scleromae

Revision editor(s): Ecsharp

Signature 2

Needs review

Curated date: 2025/07/11

Curator: Ecsharp

Revision editor(s): Ecsharp

Source: Table 2 & Section 3.3: Microbiota Analysis

Description: Description: Table 2 consists of relative abundance of specific bacterial groups in stools of BC survivors and control group by specific primers and Section 3.3. Microbiota Analysis discusses further significant relative abundance results in text. (p value of ≤ 0.05, Mann-Whitney U test)

Abundance in Group 1: decreased abundance in BC survivors

NCBI Quality ControlLinks
Akkermansia muciniphila
Bacillota
Bacteroides uniformis
Bacteroidota
Bifidobacterium
Clostridium
Clostridium perfringens
Escherichia coli
Faecalibacterium prausnitzii
Verrucomicrobiota
Akkermansia
Escherichia
Odoribacter
Parabacteroides
Propionibacterium
Streptomyces
Tannerella
Bacteroides stercoris

Revision editor(s): Ecsharp