Alternation of the gut microbiota in irritable bowel syndrome: an integrated analysis based on multicenter amplicon sequencing data/Experiment 5

From BugSigDB


Needs review

Curated date: 2025/05/01

Curator: Joiejoie

Revision editor(s): Joiejoie

Subjects

Location of subjects
Australia
Canada
Switzerland
United Kingdom
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Irritable bowel syndrome [X]Psychogenic IBS,Adaptive colitis,Colitides, Mucous,Colitis, Mucous,Colon spasm,Colon, Irritable,Functional bowel disease,IBD,IBS,IBS - Irritable bowel syndrome,IC - Irritable colon,Irritable bowel,Irritable bowel - IBS,irritable bowel syndrome,Irritable Bowel Syndromes,Irritable Colon,irritable colon,Irritable colon (disorder),Irritable colon - Irritable bowel syndrome,Irritable colon syndrome,Membranous colitis,Mucous Colitides,Mucous colitis,mucus colitis,Nervous colitis,Psychogenic IBS,psychogenic IBS,Spastic colitis,Spastic colon,spastic colon,Syndrome, Irritable Bowel,Syndromes, Irritable Bowel,Irritable bowel syndrome
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
IBS-NPD ( Irritable Bowel Syndrome without psychiatric disorders )
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group refers to patients with irritable bowel syndrome without psychiatric disorders (IBS-NPD)
Group 0 sample size Number of subjects in the control (unexposed) group
354
Group 1 sample size Number of subjects in the case (exposed) group
214

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Kruskall-Wallis
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
Matched on Factors on which subjects have been matched on in a case-control study
age, body mass index, region, sex
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age, body mass index, region, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Needs review

Curated date: 2025/05/04

Curator: Joiejoie

Revision editor(s): Joiejoie

Source: Fig. 5C

Description: The relative abundance of significantly enriched genera.

Abundance in Group 1: increased abundance in IBS-NPD ( Irritable Bowel Syndrome without psychiatric disorders )

NCBI Quality ControlLinks
Sphingobacterium
Holdemania
Enterocloster
Niallia

Revision editor(s): Joiejoie

Signature 2

Needs review

Curated date: 2025/05/04

Curator: Joiejoie

Revision editor(s): Joiejoie

Source: Fig. 5C

Description: The relative abundance of significantly depleted genera.

Abundance in Group 1: decreased abundance in IBS-NPD ( Irritable Bowel Syndrome without psychiatric disorders )

NCBI Quality ControlLinks
Acetivibrio
Acidaminococcus
Bifidobacterium
Bilophila
Butyrivibrio
Coprococcus
Corynebacterium
Faecalibacterium
Phocaeicola
Prevotella
Pseudoclostridium
Ruminiclostridium
Ruminococcus
Sarcina
Sporobacter

Revision editor(s): Joiejoie