Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-3-31

Curated date: 2025/03/18

Curator: Dorcas-od

Revision editor(s): Dorcas-od, KateRasheed

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Chronic constipation Chronic constipation,Infrequent bowel movements,chronic constipation
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal Control
Group 1 name Corresponds to the case (exposed) group for case-control studies
Functional Constipation
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients Diagnosed with Functional Constipation based on Rome IV criteria by medical doctors.
Group 0 sample size Number of subjects in the control (unexposed) group
28
Group 1 sample size Number of subjects in the case (exposed) group
30
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
ANOSIM
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Pielou Quantifies how equal the community is numerically
unchanged
Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-31

Curated date: 2025/03/19

Curator: Dorcas-od

Revision editor(s): Dorcas-od, KateRasheed

Source: Figure 1d

Description: Differential microbes of the FC and NC groups at the phylum, family, and genus levels.

Abundance in Group 1: increased abundance in Functional Constipation

NCBI Quality ControlLinks
Acidaminococcaceae
Anaerotruncus
Bacteroidaceae
Bacteroides
Bilophila
Butyricicoccus
Faecalibacterium
Flavonifractor
Hungatella
Lachnoclostridium
Marinifilaceae
Oscillibacter
Parabacteroides
Phascolarctobacterium
Roseburia
Tannerellaceae
Bacteroidota
Oscillospiraceae
Lachnospiraceae FCS020 groupLachnospiraceae FCS020 group
Ruminococcaceae UCG-002Ruminococcaceae UCG-002
Lachnospiraceae UCG-010Lachnospiraceae UCG-010
Family XIII UCG-001Family XIII UCG-001
Ruminococcaceae UCG-009Ruminococcaceae UCG-009
Faecalibacterium sp. UBA1819
Lachnospiraceae bacterium NK4A136

Revision editor(s): Dorcas-od, KateRasheed

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-3-31

Curated date: 2025/03/20

Curator: Dorcas-od

Revision editor(s): Dorcas-od, KateRasheed

Source: Figure 1D

Description: Differential microbes of the FC and NC groups at the phylum, family, and genus levels.

Abundance in Group 1: decreased abundance in Functional Constipation

NCBI Quality ControlLinks
Blautia
Citrobacter
Eggerthella
Enterobacter
Enterobacteriaceae
Gordonibacter
Lachnospiraceae
Mesorhizobium
Peptostreptococcaceae
Pseudomonadaceae
Pseudomonadota
Pseudomonas
Rhizobiaceae
Romboutsia
Mediterraneibacter gnavus

Revision editor(s): Dorcas-od, KateRasheed