Changes in the gut microbiota structure and function in rats with doxorubicin-induced heart failure/Experiment 9
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-7-14
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Heart failure cardiac failure,cardiac insufficiency,failure, heart,heart failure,Heart failure (disorder),Heart failure NOS,Heart failure NOS (disorder),Heart failure, unspecified,Heart: [weak] or [failure NOS],insufficiency, Cardiac,Weak heart,Heart failure
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control (Con-K) group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Doxorubicin-I (Dox-I) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The rats in the DOX-I group were administered doxorubicin intraperitoneally in the following concentrations: 4mg/kg in weeks 1, 3, and 5, and 2mg/kg in weeks 2, 4 & 6 to induce heart failure, and their body weights increased to varying degrees.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 9
- Group 1 sample size Number of subjects in the case (exposed) group
- 7
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-14
Source: Figure 8 & 9
Description: Histogram and Cladogram analyzed by LEfSe (LDA = 4.0, P < 0.05) showing the phylogenetic distribution of the bacterial lineages in the CON group and DOX group.
Abundance in Group 1: increased abundance in Doxorubicin-I (Dox-I) group
Revision editor(s): Victoria
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-14
Source: Figure 8 & 9
Description: Histogram and Cladogram analyzed by LEfSe (LDA = 4.0, P < 0.05) showing the phylogenetic distribution of the bacterial lineages in the CON group and DOX group.
Abundance in Group 1: decreased abundance in Doxorubicin-I (Dox-I) group
Revision editor(s): Victoria
