Protective effect of L-pipecolic acid on constipation in C57BL/6 mice based on gut microbiome and serum metabolomic/Experiment 1

From BugSigDB


Needs review

Curated date: 2025/03/14

Curator: Mautin

Revision editor(s): Mautin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Constipation Constipation,Costiveness,Dyschezia,constipation
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy Controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
Functional Constipation(FC)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
children/patients with functional constipation, with infrequent bowel movements and who fulfilled the Roman IV diagnostic criteria
Group 0 sample size Number of subjects in the control (unexposed) group
28
Group 1 sample size Number of subjects in the case (exposed) group
26
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased

Signature 1

Needs review

Curated date: 2025/03/16

Curator: Mautin

Revision editor(s): Mautin

Source: Figure 1F

Description: The Differential analysis between healthy Controls and Functional constipation using STAMP Analysis

Abundance in Group 1: increased abundance in Functional Constipation(FC)

NCBI Quality ControlLinks
Acetanaerobacterium
Eubacterium
Intestinimonas
Klebsiella
Ruminiclostridium
Ruminococcaceae UCG-005Ruminococcaceae UCG-005
Ruminococcaceae NK4A214Ruminococcaceae NK4A214
Ruminiclostridium 9Ruminiclostridium 9
Lachnospiraceae UCG-010Lachnospiraceae UCG-010
Ruminococcaceae UCG-004Ruminococcaceae UCG-004

Revision editor(s): Mautin

Signature 2

Needs review

Curated date: 2025/03/16

Curator: Mautin

Revision editor(s): Mautin

Source: Figure 1F

Description: The Differential analysis between healthy Controls and Functional constipation using STAMP Analysis

Abundance in Group 1: decreased abundance in Functional Constipation(FC)

NCBI Quality ControlLinks
Bacillus
Gemella
Halomonas
Ochrobactrum
Phascolarctobacterium
Phenylobacterium

Revision editor(s): Mautin