Integrated fecal microbiota and metabolomics analysis of the orlistat intervention effect on polycystic ovary syndrome rats induced by letrozole combined with a high-fat diet/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment outcome measurement Treatment outcome measurement,treatment outcome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- PCOS (Polycystic ovary syndrome ) Control group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- ORL-PCOS (Orlistat-Polycystic ovary syndrome) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The ORL-PCOS (Orlistat-Polycystic ovary syndrome) group was treated with different doses of orlistat (low-20 mg/kg/d; medium-40 mg/kg/d; high-80 mg/kg/d; Lunan Pharmaceutical Group Corporation; Shandong, Linyi, China.) over the next 12 weeks.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Curated date: 2025/04/30
Curator: PreciousChijioke
Revision editor(s): PreciousChijioke, Victoria, KateRasheed
Source: Fig. 4A and 4B
Description: LEfSe of the gut microbiota of the ORL-PCOS and PCOS groups.
Abundance in Group 1: increased abundance in ORL-PCOS (Orlistat-Polycystic ovary syndrome) group
Revision editor(s): PreciousChijioke, Victoria, KateRasheed
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Curated date: 2025/05/01
Curator: PreciousChijioke
Revision editor(s): PreciousChijioke, KateRasheed
Source: Fig. 4A and 4B
Description: LEfSe of the gut microbiota of the ORL-PCOS and PCOS groups.
Abundance in Group 1: decreased abundance in ORL-PCOS (Orlistat-Polycystic ovary syndrome) group
Revision editor(s): PreciousChijioke, KateRasheed
