Oral and gut microbial biomarkers of susceptibility to respiratory tract infection in adults: A feasibility study/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-3-31

Curated date: 2025/03/22

Curator: UtibeIta

Revision editor(s): UtibeIta

Subjects

Location of subjects
United Kingdom
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Respiratory tract infectious disease infection, respiratory tract,infections, respiratory,infections, respiratory tract,infections, upper respiratory,infections, upper respiratory tract,respiratory infection, upper,respiratory infections,respiratory tract infection,upper respiratory infections,upper respiratory tract infections,Respiratory tract infectious disease,respiratory tract infectious disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
respiratory tract infection(baseline)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
participants who acquired Respiratory Tract Infection symptoms at the baseline/start of the study
Group 0 sample size Number of subjects in the control (unexposed) group
21
Group 1 sample size Number of subjects in the case (exposed) group
19

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-31

Curated date: 2025/03/25

Curator: UtibeIta

Revision editor(s): UtibeIta

Source: Figure 5

Description: Difference between microbial biomarkers in gut baseline samples from participants who acquired Respiratory Tract Infection symptoms compared to those who remained healthy.

Abundance in Group 1: increased abundance in respiratory tract infection(baseline)

NCBI Quality ControlLinks
Eggerthella sp.
Enhydrobacter
Enhydrobacter sp.
Lysobacteraceae
Lysobacterales
Rikenellaceae
Veillonella sp.

Revision editor(s): UtibeIta

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-3-31

Curated date: 2025/03/25

Curator: UtibeIta

Revision editor(s): UtibeIta

Source: Figure 5

Description: Difference between microbial biomarkers in gut baseline samples from participants who acquired Respiratory Tract Infection symptoms compared to those who remained healthy.

Abundance in Group 1: decreased abundance in respiratory tract infection(baseline)

NCBI Quality ControlLinks
Coprobacillus sp.
Desulfobacterales
Desulfobulbaceae
Desulfobulbus
Desulfobulbus sp.

Revision editor(s): UtibeIta