The Human Ocular Surface Microbiome and Its Associations with the Tear Proteome in Dry Eye Disease/Experiment 2
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Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-22
Curated date: 2023/10/01
Curator: Mary Bearkland
Revision editor(s): Mary Bearkland, ChiomaBlessing, WikiWorks
Subjects
- Location of subjects
- Switzerland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Palpebral conjunctiva Conjunctival layer of eyelids,Eyelid conjunctiva,Tarsal conjunctiva,Tunica conjunctiva palpebrum,Palpebral conjunctiva,palpebral conjunctiva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Dry eye syndrome dry eye,Dry Eye Syndrome,dry eye syndrome,Dry Eye Syndromes,dry eye(s),eye(s), dry,KCS,Keraconjunctivitis sicca,Keratoconjunctivitis Sicca,Keratoconjunctivitis sicca,keratoconjunctivitis sicca,Keratoconjunctivitis sicca (disorder),sicca, keratoconjunctivitis,Tear film insufficiency,tear film insufficiency,Dry eye syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Dry Eye
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Dry Eye Disease diagnosis
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Antibiotics within the past 3 months
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Welch's T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Signature 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-22
Source: Figure 4a
Description: Figure 4. The distinct taxonomical composition of the ocular surface microbiome between DED patients and healthy controls. The relative abundances of taxa (c) associated with DED (Mann–Whitney test). Mean values and standard deviations are shown.
Abundance in Group 1: increased abundance in Dry Eye
| NCBI | Quality Control | Links |
|---|---|---|
| Corynebacterium tuberculostearicum |
Revision editor(s): Mary Bearkland, WikiWorks
