Rifaximin Ameliorates Loperamide-Induced Constipation in Rats through the Regulation of Gut Microbiota and Serum Metabolites/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-4-10

Curated date: 2025/04/02

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Rattus norvegicus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to antibiotic Response to antibiotic,response to antibiotic
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of LOP (loperamide) and RIF (rifaximin) group
Group 1 name Corresponds to the case (exposed) group for case-control studies
CTR (control) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Rats in this group were injected with 0.9% saline.
Group 0 sample size Number of subjects in the control (unexposed) group
14
Group 1 sample size Number of subjects in the case (exposed) group
7

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3.5


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-10

Curated date: 2025/04/03

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 3G and 3H

Description: Histogram of LDA value distribution generated by LEfSe indicating the differences of bacterial communities among three groups after LDA using a threshold score of LDA > 3.5

Abundance in Group 1: increased abundance in CTR (control) group

NCBI Quality ControlLinks
Candidatus Altimarinota
Candidatus Saccharimonadaceae
Candidatus Saccharimonadales
Candidatus Saccharimonadia
Candidatus Saccharimonas
Lactobacillus intestinalis
Ligilactobacillus murinus
Rikenellaceae
Clostridia UCG-014Clostridia UCG-014
Alistipes

Revision editor(s): Tosin