Multi-omics analysis of fecal microbiota transplantation's impact on functional constipation and comorbid depression and anxiety
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Yang C, Hu T, Xue X, Su X, Zhang X, Fan Y, Shen X, Dong X
Journal
BMC microbiology
Year
2023
Keywords:
5-hydroxytryptamine, Constipation, Depression and anxiety, Fecal microbiota transplantation, Metabolomics, Metagenomics
BACKGROUND: Depression and anxiety are common comorbid diseases of constipation. Fecal microbiota transplantation (FMT) significantly relieves gastrointestinal-related symptoms, but its impact on psychiatric symptoms remains uncharted. METHODS: We collected fecal and serum samples before and after FMT from 4 functional constipation patients with psychiatric symptoms and corresponding donor stool samples. We categorized the samples into two groups: before FMT (Fb) and after FMT (Fa). Parameters associated with constipation, depression, and anxiety symptoms were evaluated. Metagenomics and targeted neurotransmitter metabolomics were performed to investigate the gut microbiota and metabolites. 5-hydroxytryptamine (5-HT) biosynthesis was detected in patients' fecal supernatants exposed to the QGP-1 cell model in vitro. RESULTS: Our study demonstrated that patient's constipation, depression, and anxiety were improved after FMT intervention. At the genus level, relative abundance of g_Bacteroides and g_Klebsiella decreased in the Fa group, while g_Lactobacillus, and g_Selenomonas content increased in the same group. These observations suggest a potential involvement of these genera in the pathogenesis of constipation with psychiatric symptoms. Metabolomics analysis showed that FMT intervention decreased serum 5-HT levels. Additionally, we found that species, including s_Klebsiella sp. 1_1_55, s_Odoribacter splanchnicus, and s_Ruminococcus gnavus CAG:126, were positively correlated with 5-HT levels. In contrast, s_Acetobacterium bakii, s_Enterococcus hermanniensis, s_Prevotella falsenii, s_Propionispira arboris, s_Schwartzia succinivorans, s_Selenomonas artemidis, and s_Selenomonas sp. FC4001 were negatively correlated with 5-HT levels. Furthermore, we observed that patients' fecal supernatants increased 5-HT biosynthesis in QGP-1 cells. CONCLUSION: FMT can relieve patients' constipation, depression, and anxiety symptoms by reshaping gut microbiota. The 5-HT level was associated with an altered abundance of specific bacteria or metabolites. This study provides specific evidence for FMT intervention in constipation patients with psychiatric symptoms.
Experiment 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to transplant Response to transplant,response to transplant
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Fb (Patients before FMT - Fecal microbiota transplantation)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Fa (Patients After FMT - Fecal microbiota transplantation)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with functional constipation and comorbid depression/anxiety after undergoing fecal microbiota transplantation (FMT).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 4
- Group 1 sample size Number of subjects in the case (exposed) group
- 4
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 weeks
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Source: Fig 2C
Description: The shift in gut microbiota before and after FMT based on metagenomic sequencing data.
Abundance in Group 1: increased abundance in Fa (Patients After FMT - Fecal microbiota transplantation)
| NCBI | Quality Control | Links |
|---|---|---|
| Enterococcus hermanniensis |
Revision editor(s): Shulamite, Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Source: Fig 2E
Description: The shift in gut microbiota before and after FMT based on metagenomic sequencing data.
Abundance in Group 1: decreased abundance in Fa (Patients After FMT - Fecal microbiota transplantation)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides pyogenes |
Revision editor(s): Shulamite, Anne-mariesharp
Experiment 4
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Differences from previous experiment shown
Subjects
Lab analysis
Statistical Analysis
- Statistical test
- Mann-Whitney (Wilcoxon)
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Source: Table S2 and Table 3
Description: Differential relative abundance of species between the Fb and Fa groups
Abundance in Group 1: increased abundance in Fa (Patients After FMT - Fecal microbiota transplantation)
Revision editor(s): Shulamite, Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-16
Source: Table S2 and Table 3
Description: Differential relative abundance of species between the Fb and Fa groups
Abundance in Group 1: decreased abundance in Fa (Patients After FMT - Fecal microbiota transplantation)
Revision editor(s): Shulamite, Anne-mariesharp
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