Gut microbiota affects the estrus return of sows by regulating the metabolism of sex steroid hormones/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-6-11

Curated date: 2025/04/25

Curator: Victoria

Revision editor(s): Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Sus scrofa domesticus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Sex hormone Geschlechtshormon,Geschlechtshormone,hormone sexuelle,hormones sexuelles,sex hormones,Sexualhormon,Sexualhormone,Sex hormone,sex hormone
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non-return sows (Discovery cohort)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Normal Return sows (Discovery cohort)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group comprises sows that returned to estrus within 7 days of weaning in the discovery cohort.
Group 0 sample size Number of subjects in the control (unexposed) group
40
Group 1 sample size Number of subjects in the case (exposed) group
167

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-11

Curated date: 2025/04/25

Curator: Victoria

Revision editor(s): Victoria

Source: Additional file 1: Figure S4 D&E, Additional file2: Table S2

Description: Differential ASVs and genera between normal return and non-return sows in 207 fecal samples.

Abundance in Group 1: increased abundance in Normal Return sows (Discovery cohort)

NCBI Quality ControlLinks
Bacteroidales
Lactobacillus johnsonii
Muribaculaceae
Prevotella
Ruminococcaceae bacterium UCG-005
Candidatus Alcium sp. F082
uncultured eubacterium WCHB1-41
Bacteroidales RF16 groupBacteroidales RF16 group
Rikenellaceae RC9 gut groupRikenellaceae RC9 gut group
Lachnospiraceae XPB1014 groupLachnospiraceae XPB1014 group
Prevotellaceae UCG-003Prevotellaceae UCG-003

Revision editor(s): Victoria

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-6-11

Curated date: 2025/04/25

Curator: Victoria

Revision editor(s): Victoria, KateRasheed

Source: Additional file 1: Figure S4 D&E, Additional file2: Table S2

Description: Differential ASVs and genera between normal return and non-return sows in 207 fecal samples.

Abundance in Group 1: decreased abundance in Normal Return sows (Discovery cohort)

NCBI Quality ControlLinks
Bacteroides
Bacteroides fragilis
Bifidobacterium
Bifidobacterium pseudolongum
Cellulosilyticum
Clostridium
Clostridium bornimense
Clostridium perfringens
Clostridium septicum
Enterococcus
Eubacterium coprostanoligenes
Lachnospiraceae
Lachnospiraceae bacterium XBB1006
Mogibacterium sp.
Negativibacillus
Oscillospiraceae
Parabacteroides
Peptococcaceae
Phocaeicola plebeius
Streptococcus
Terrisporobacter
Rikenellaceae RC9 gut groupRikenellaceae RC9 gut group
Christensenellaceae R-7 groupChristensenellaceae R-7 group
Clostridia UCG-014Clostridia UCG-014
NK4A214 groupNK4A214 group
Family XIII AD3011 groupFamily XIII AD3011 group
Clostridium sensu stricto 6Clostridium sensu stricto 6
Ruminococcaceae bacterium UCG-005

Revision editor(s): Victoria, KateRasheed